PMID- 21904247
OWN - NLM
STAT- MEDLINE
DCOM- 20120723
LR  - 20201209
IS  - 1530-0315 (Electronic)
IS  - 0195-9131 (Linking)
VI  - 44
IP  - 4
DP  - 2012 Apr
TI  - Chocolate milk and endurance exercise recovery: protein balance, glycogen, and 
      performance.
PG  - 682-91
LID - 10.1249/MSS.0b013e3182364162 [doi]
AB  - PURPOSE: This study examined effects of fat-free chocolate milk (MILK) 
      consumption on kinetic and cellular markers of protein turnover, muscle glycogen, 
      and performance during recovery from endurance exercise. METHODS: Male runners 
      participated in two trials separated by 1 wk and consumed either MILK or a 
      nonnitrogenous isocaloric carbohydrate (CHO) control beverage (CON) after a 
      45-min run at 65% of V O(2peak). Postexercise muscle protein fractional synthetic 
      rate (FSR) and whole-body protein turnover were determined during 3 h of recovery 
      using muscle biopsies and primed constant infusions of L-[ring-(2)H(5)]phenylalanine 
      and L-[1-(1)(3)C]leucine, respectively. Phosphorylation of translational signaling 
      proteins and activity of proteolytic molecules were determined using Western 
      blotting and enzymatic activity assays. Muscle glycogen was quantified, and 
      treadmill time to exhaustion was determined after the recovery period. RESULTS: 
      Consuming MILK after exercise resulted in higher mixed muscle FSR with lower 
      whole-body proteolysis and synthesis compared with CON (P </= 0.05). 
      Phosphorylation of eIF4E-BP1 and FOXO3a was higher for MILK (P < 0.01), whereas 
      Akt phosphorylation was lower during recovery regardless of dietary treatment (P 
      < 0.05). Enzymatic activity assays indicated lower caspase-3 activity during 
      recovery for MILK (P < 0.01) and higher 26S proteasome activity for CON (P < 
      0.01). Muscle glycogen was not affected by either dietary treatment; however, 
      time to exhaustion was greater for MILK than for CON (P < 0.05). CONCLUSIONS: The 
      effects of consumption of MILK after endurance exercise on FSR, signaling 
      molecules of skeletal muscle protein turnover, leucine kinetics, and performance 
      measures suggest unique benefits of milk compared with a CHO-only beverage.
FAU - Lunn, William R
AU  - Lunn WR
AD  - Exercise Science Department, Southern Connecticut State University, Moore Field 
      House, Room 169, New Haven, CT 06515, USA. lunnw1@southernct.edu
FAU - Pasiakos, Stefan M
AU  - Pasiakos SM
FAU - Colletto, Megan R
AU  - Colletto MR
FAU - Karfonta, Kirstin E
AU  - Karfonta KE
FAU - Carbone, John W
AU  - Carbone JW
FAU - Anderson, Jeffrey M
AU  - Anderson JM
FAU - Rodriguez, Nancy R
AU  - Rodriguez NR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Med Sci Sports Exerc
JT  - Medicine and science in sports and exercise
JID - 8005433
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (EIF4EBP1 protein, human)
RN  - 0 (FOXO3 protein, human)
RN  - 0 (Forkhead Box Protein O3)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Muscle Proteins)
RN  - 0 (Phosphoproteins)
RN  - 9005-79-2 (Glycogen)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
RN  - EC 3.4.99.- (ATP dependent 26S protease)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/metabolism
MH  - Adult
MH  - Animals
MH  - Athletic Performance/*physiology
MH  - Cacao/*metabolism
MH  - Cell Cycle Proteins
MH  - Dietary Carbohydrates/metabolism
MH  - Forkhead Box Protein O3
MH  - Forkhead Transcription Factors/metabolism
MH  - Glycogen/analysis
MH  - Heart Rate/physiology
MH  - Humans
MH  - Male
MH  - Milk/*metabolism
MH  - Muscle Proteins/*biosynthesis
MH  - Muscle, Skeletal/chemistry/metabolism/physiology
MH  - Oxygen Consumption/physiology
MH  - Phosphoproteins/metabolism
MH  - Phosphorylation
MH  - Physical Endurance/*physiology
MH  - Proteasome Endopeptidase Complex/analysis
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Young Adult
EDAT- 2011/09/10 06:00
MHDA- 2012/07/24 06:00
CRDT- 2011/09/10 06:00
PHST- 2011/09/10 06:00 [entrez]
PHST- 2011/09/10 06:00 [pubmed]
PHST- 2012/07/24 06:00 [medline]
AID - 10.1249/MSS.0b013e3182364162 [doi]
PST - ppublish
SO  - Med Sci Sports Exerc. 2012 Apr;44(4):682-91. doi: 10.1249/MSS.0b013e3182364162.