PMID- 21905956
OWN - NLM
STAT- MEDLINE
DCOM- 20120201
LR  - 20181201
IS  - 1477-2566 (Electronic)
IS  - 1465-3249 (Linking)
VI  - 13
IP  - 10
DP  - 2011 Nov
TI  - Immunoregulatory effects of human dental pulp-derived stem cells on T cells: 
      comparison of transwell co-culture and mixed lymphocyte reaction systems.
PG  - 1205-20
LID - 10.3109/14653249.2011.605351 [doi]
AB  - BACKGROUND AIMS. Studies performed using human and animal models have indicated 
      the immunoregulatory capability of mesenchymal stromal cells in several lineages. 
      We investigated whether human dental pulp-derived stem cells (hDP-SC) have 
      regulatory effects on phytohemagglutinin (PHA)-activated CD3(+) T cells. We aimed 
      to define the regulatory mechanisms associated with hDP-SC that occur in mixed 
      lymphocyte reaction (MLR) and transwell systems with PHA-CD3(+) T cells and 
      hDP-SC at a ratio of 1:1. METHODS. Proliferation, apoptosis and pro- and 
      anti-inflammatory cytokines of PHA-CD3(+)T cells, the expression of Regulatory T 
      cells (Treg) markers and some regulatory factors related to hDP-SC, were studied 
      in Both transwell and MLR are co-cultures systems. RESULTS. Anti-proliferative 
      and apoptotic effects of hDP-SC were determined in co-culture systems. Elevated 
      expression levels of human leukocyte antigen (HLA)-G, hepatocyte growth factor 
      (HGF)-beta1, intracellular adhesion molecule (ICAM-1)-1, interleukin (IL)-6, IL-10, 
      transforming growth factor (TGF)-beta1, vascular adhesion molecule (VCAM)-1 and 
      vascular endothelial growth factor (VEGF) by hDP-SC were detected in the 
      co-culture systems. We observed decreased expression levels of pro-inflammatory 
      cytokines [interferon (IFN)-gamma, IL-2, IL-6 receptor (R), IL-12, Interleukin-17A 
      (IL-17A), tumor necrosis factor (TNF)-alpha] and increased expression levels of 
      anti-inflammatory cytokine [inducible protein (IP)-10] from PHA-CD3(+) T cells in 
      the transwell system. Expression of Treg (CD4(+) CD25(+) Foxp3(+)) markers was 
      significantly induced by hDP-SC in both co-culture systems. We observed apoptosis 
      of PHA-CD3(+) T cells with 24 h using time-lapse camera photographs and active 
      caspase labeling; it is likely that paracrine soluble factors and molecular 
      signals secreted by hDP-SC led this apoptosis. CONCLUSIONS. We suggest that 
      hDP-SC have potent immunoregulatory functions because of their soluble factors 
      and cytokines via paracrine mechanisms associated with PHA-CD3(+) T cells, which 
      could contribute to clinical therapies.
FAU - Demircan, Pinar Cetinalp
AU  - Demircan PC
AD  - Center for Stem Cell and Gene Therapies Research and Practice, Institute of 
      Health Sciences, Stem Cell Department, Kocaeli University, Turkey.
FAU - Sariboyaci, Ayla Eker
AU  - Sariboyaci AE
FAU - Unal, Zehra Seda
AU  - Unal ZS
FAU - Gacar, Gulcin
AU  - Gacar G
FAU - Subasi, Cansu
AU  - Subasi C
FAU - Karaoz, Erdal
AU  - Karaoz E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110912
PL  - England
TA  - Cytotherapy
JT  - Cytotherapy
JID - 100895309
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Cytokines)
SB  - IM
MH  - Adult
MH  - Adult Stem Cells/cytology/immunology/*metabolism
MH  - Antigens, Differentiation/metabolism
MH  - Apoptosis
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Cytokines/metabolism
MH  - Dental Pulp/*cytology
MH  - Humans
MH  - Immunomodulation
MH  - Lymphocyte Culture Test, Mixed
MH  - Male
MH  - Mesenchymal Stem Cells/cytology/immunology/*metabolism
MH  - Paracrine Communication
MH  - Stem Cell Niche
MH  - T-Lymphocyte Subsets/cytology/immunology/*metabolism
MH  - T-Lymphocytes, Regulatory/cytology/immunology/*metabolism
EDAT- 2011/09/13 06:00
MHDA- 2012/02/02 06:00
CRDT- 2011/09/13 06:00
PHST- 2011/09/13 06:00 [entrez]
PHST- 2011/09/13 06:00 [pubmed]
PHST- 2012/02/02 06:00 [medline]
AID - S1465-3249(11)70601-6 [pii]
AID - 10.3109/14653249.2011.605351 [doi]
PST - ppublish
SO  - Cytotherapy. 2011 Nov;13(10):1205-20. doi: 10.3109/14653249.2011.605351. Epub 
      2011 Sep 12.