PMID- 21906041
OWN - NLM
STAT- MEDLINE
DCOM- 20120105
LR  - 20110912
IS  - 1940-9818 (Electronic)
IS  - 0736-6205 (Linking)
VI  - 51
IP  - 3
DP  - 2011 Sep
TI  - The fluorescent dyes TO-PRO-3 and TOTO-3 iodide allow detection of microbial 
      cells in soil samples without interference from background fluorescence.
PG  - 190-2
LID - 10.2144/000113736 [doi]
AB  - Visualization of microorganisms in soils and sediments using fluorescent dyes is 
      a common method in microbial ecology studies, but is often hampered by strong 
      nonspecific background fluorescence that can mask genuine cellular signals. The 
      cyanine nucleic acid binding dyes TO-PRO-3 and TOTO-3 iodide enabled a clear 
      detection of microbial cells in a mineral soil, while nonspecific background was 
      greatly reduced compared with commonly used dyes. When used as counterstains for 
      fluorescence in situ hybridization (FISH), both cyanine dyes allowed 
      identification of microbial cells despite strong background from nonspecifically 
      bound probes. TO-PRO-3 and TOTO-3 are easy to use and represent superior 
      alternatives for detecting microorganisms in soil environments.
FAU - Henneberger, Ruth
AU  - Henneberger R
AD  - Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, 
      NSW 2109, Australia.
FAU - Birch, Debra
AU  - Birch D
FAU - Bergquist, Peter
AU  - Bergquist P
FAU - Walter, Malcolm
AU  - Walter M
FAU - Anitori, Roberto P
AU  - Anitori RP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biotechniques
JT  - BioTechniques
JID - 8306785
RN  - 0 (Carbocyanines)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Quinolines)
RN  - 0 (TO-PRO-3)
RN  - 0 (TOTO-3 compound)
RN  - 0 (Thiazoles)
SB  - IM
MH  - Bacteria/*isolation & purification
MH  - Carbocyanines/*analysis/chemistry
MH  - Fluorescent Dyes/*analysis/chemistry
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Quinolines/*analysis/chemistry
MH  - *Soil Microbiology
MH  - Thiazoles/*analysis/chemistry
EDAT- 2011/09/13 06:00
MHDA- 2012/01/06 06:00
CRDT- 2011/09/13 06:00
PHST- 2011/05/12 00:00 [received]
PHST- 2011/08/12 00:00 [accepted]
PHST- 2011/09/13 06:00 [entrez]
PHST- 2011/09/13 06:00 [pubmed]
PHST- 2012/01/06 06:00 [medline]
AID - 000113736 [pii]
AID - 10.2144/000113736 [doi]
PST - ppublish
SO  - Biotechniques. 2011 Sep;51(3):190-2. doi: 10.2144/000113736.