PMID- 21907460
OWN - NLM
STAT- MEDLINE
DCOM- 20120615
LR  - 20211020
IS  - 1558-1497 (Electronic)
IS  - 0197-4580 (Print)
IS  - 0197-4580 (Linking)
VI  - 33
IP  - 4
DP  - 2012 Apr
TI  - Aging and infection reduce expression of specific brain-derived neurotrophic 
      factor mRNAs in hippocampus.
PG  - 832.e1-14
LID - 10.1016/j.neurobiolaging.2011.07.015 [doi]
AB  - Aging increases the likelihood of cognitive decline after negative life events 
      such as infection or injury. We have modeled this increased vulnerability in aged 
      (24-month-old), but otherwise unimpaired F344xBN rats. In these animals, but not 
      in younger (3-month-old) counterparts, a single intraperitoneal injection of E. 
      coli leads to specific deficits in long-term memory and long-lasting synaptic 
      plasticity in hippocampal area CA1-processes strongly dependent on brain-derived 
      neurotrophic factor (BDNF). Here we have investigated the effects of age and 
      infection on basal and fear-conditioning-stimulated expression of Bdnf in 
      hippocampus. We performed in situ hybridization with 6 probes recognizing: total 
      (pan-)BDNF mRNA, the 4 predominant 5' exon-specific transcripts (I, II, IV, and 
      VI), and BDNF mRNAs with a long 3' untranslated region (3' UTR). In CA1, aging 
      reduced basal levels and fear-conditioning-induced expression of total BDNF mRNA, 
      exon IV-specific transcripts, and transcripts with long 3' UTRs; effects of 
      infection were similar and sometimes compounded the effects of aging. In CA3, 
      aging reduced all of the transcripts to some degree; infection had no effect. 
      Effects in dentate were minimal. Northern blot analysis confirmed an 
      aging-associated loss of total BDNF mRNA in areas CA1 and CA3, and revealed a 
      parallel, preferential loss of BDNF mRNA transcripts with long 3' UTRs.
CI  - Copyright A(c) 2012 Elsevier Inc. All rights reserved.
FAU - Chapman, Timothy R
AU  - Chapman TR
AD  - Department of Psychology and Neuroscience and The Center for Neuroscience, 
      University of Colorado, Boulder, CO 80309, USA.
FAU - Barrientos, Ruth M
AU  - Barrientos RM
FAU - Ahrendsen, Jared T
AU  - Ahrendsen JT
FAU - Hoover, Jennifer M
AU  - Hoover JM
FAU - Maier, Steven F
AU  - Maier SF
FAU - Patterson, Susan L
AU  - Patterson SL
LA  - eng
GR  - R01 AG028271-04/AG/NIA NIH HHS/United States
GR  - R01 AG028271/AG/NIA NIH HHS/United States
GR  - 1R21AG031467/AG/NIA NIH HHS/United States
GR  - R21 AG031467/AG/NIA NIH HHS/United States
GR  - R21 AG031467-01A2/AG/NIA NIH HHS/United States
GR  - 1R01AG02827/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110909
PL  - United States
TA  - Neurobiol Aging
JT  - Neurobiology of aging
JID - 8100437
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - Age Factors
MH  - Aging/*pathology
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Disease Models, Animal
MH  - Escherichia coli Infections/*pathology
MH  - Exons/genetics
MH  - Fear/psychology
MH  - Gene Expression Regulation/*physiology
MH  - Hippocampus/*metabolism
MH  - Male
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Rats, Inbred F344
PMC - PMC3237944
MID - NIHMS316871
COIS- Disclosure Statement: None of the authors have actual or potential conflicts of 
      interest with the work reported here.
EDAT- 2011/09/13 06:00
MHDA- 2012/06/16 06:00
PMCR- 2013/04/01
CRDT- 2011/09/13 06:00
PHST- 2011/02/16 00:00 [received]
PHST- 2011/07/13 00:00 [revised]
PHST- 2011/07/28 00:00 [accepted]
PHST- 2011/09/13 06:00 [entrez]
PHST- 2011/09/13 06:00 [pubmed]
PHST- 2012/06/16 06:00 [medline]
PHST- 2013/04/01 00:00 [pmc-release]
AID - S0197-4580(11)00300-9 [pii]
AID - 10.1016/j.neurobiolaging.2011.07.015 [doi]
PST - ppublish
SO  - Neurobiol Aging. 2012 Apr;33(4):832.e1-14. doi: 
      10.1016/j.neurobiolaging.2011.07.015. Epub 2011 Sep 9.