PMID- 2191015
OWN - NLM
STAT- MEDLINE
DCOM- 19900717
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 28
IP  - 5
DP  - 1990 May
TI  - Immunodetection of human T-cell lymphotropic virus type I core protein in 
      biological samples by using a monoclonal antibody immunoassay.
PG  - 949-55
AB  - A monoclonal antibody-based enzyme immunoassay (EIA) has been developed for 
      detection of human T-cell lymphotropic virus type I (HTLV-I) core protein. The 
      monoclonal antibody (clone 6.11) specifically recognizes the p19 gag gene-encoded 
      protein of the virus. The EIA was over 100 times more sensitive than reverse 
      transcriptase measurement and was capable of responding to less than 500 pg of 
      whole-virus lysate. The assay exhibited type specificity in that HTLV-II antigens 
      failed to produce a positive signal. In addition, a panel of other viruses 
      demonstrated no antigenic cross-reactivity. These included herpesviruses, measles 
      virus, human immunodeficiency viruses, and others. Viral p19 was followed during 
      the course of density gradient ultracentrifugation in the presence of detergent, 
      where it was noted to associate with viral membrane proteins. In comparison, 
      reverse transcriptase activity localized in fractions of higher density 
      containing envelope-free cores. Of clinical interest, the EIA was used to detect 
      HTLV-I antigen in the viral cultures of patients with HTLV-I-associated 
      myelopathies and from symptom-free individuals with proviral integration.
FAU - Papsidero, L
AU  - Papsidero L
AD  - Department of Medicine, State University of New York, Syracuse 13210.
FAU - Swartzwelder, F
AU  - Swartzwelder F
FAU - Sheu, M
AU  - Sheu M
FAU - Montagna, R
AU  - Montagna R
FAU - Ehrlich, G
AU  - Ehrlich G
FAU - Bhagavati, S
AU  - Bhagavati S
FAU - Dosik, H
AU  - Dosik H
FAU - Sninsky, J
AU  - Sninsky J
FAU - Poiesz, B
AU  - Poiesz B
LA  - eng
GR  - N01HB67021/HB/NHLBI NIH HHS/United States
GR  - R01HL43602-01/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Viral)
RN  - 0 (Gene Products, gag)
RN  - 0 (Retroviridae Proteins, Oncogenic)
RN  - 0 (Viral Core Proteins)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antigens, Viral/analysis
MH  - Gene Products, gag/analysis
MH  - HTLV-I Infections/diagnosis/immunology
MH  - Human T-lymphotropic virus 1/*analysis/immunology
MH  - Humans
MH  - *Immunoenzyme Techniques
MH  - Retroviridae Proteins, Oncogenic/*analysis
MH  - Viral Core Proteins/*analysis
PMC - PMC267844
EDAT- 1990/05/01 00:00
MHDA- 1990/05/01 00:01
PMCR- 1990/05/01
CRDT- 1990/05/01 00:00
PHST- 1990/05/01 00:00 [pubmed]
PHST- 1990/05/01 00:01 [medline]
PHST- 1990/05/01 00:00 [entrez]
PHST- 1990/05/01 00:00 [pmc-release]
AID - 10.1128/jcm.28.5.949-955.1990 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1990 May;28(5):949-55. doi: 10.1128/jcm.28.5.949-955.1990.