PMID- 21910346
OWN - NLM
STAT- MEDLINE
DCOM- 20130801
LR  - 20161018
IS  - 1003-5370 (Print)
IS  - 1003-5370 (Linking)
VI  - 31
IP  - 8
DP  - 2011 Aug
TI  - [Anticancer effect of 5-fluorouracil combined with extract of Rosa roxburghii 
      Tratt on human endometrial adenocarcinoma].
PG  - 1108-12, 1117
AB  - OBJECTIVE: To investigate anticancer effects of 5-fluorouracil (5-FU) combined 
      with CL, extract of Rosa roxburghii Tratt on human endometrial adenocarcinoma 
      cell line (JEC). METHODS: JEC cells cultured in vitro in the logarithmic growth 
      phase were seeded in the culture plate and divided into the control group (RPMI 
      1640), the positive group (10(-4) mol/L 5-FU), the CL groups (at the dose of 
      0.01, 0.1, 1, 10, and 100 microg/mL), and the CL (0.01, 0.1, 1, 10, and 100 
      microg/mL) combined with 5-FU groups. Effects of 5-FU combined with CL on JEC 
      cell growth were drawn and measured by MTT and growth curves. Effects of CL 
      combined with 5-FU on the JEC cell differentiation was analyzed by detecting the 
      reduction capability of nitrobenzene thiocyanate (NBT) and lactate dehydrogenase 
      (LDH) contents in the cultured medium. Effects of CL combined with 5-FU on the 
      JEC cell apoptosis and cell proliferation cycle were detected by acridine orange 
      (AO)/ethidium bromide (EB) fluorescent staining and flow cytometry (FCM). 
      RESULTS: The proliferation inhibitory effect of CL combined with 5-FU on JEC 
      cells was enhanced when compared with that of CL or 5-FU alone (P<0.05). The 
      percentages of NBT positive JEC cells and apoptotic JEC cells increased in the 
      5-FU combined with CL groups when compared with 5-FU group or the CL group alone 
      (P<0.05). The LDH concentration of the JEC cell culture supernate decreased in 
      5-FU combined with CL groups (P<0.05). Furthermore, the percentage of G0-G1 phase 
      JEC cells treated by 5-FU combined with CL was higher than that of 5-FU or CL 
      alone (P<0.05). CONCLUSION: CL could enhance anticancer effects of 5-FU. Its 
      mechanisms might be correlated with reinforcing the cytotoxicity of 5-FU, 
      inducing cell differentiation and apoptosis, and inhibiting cell proliferation 
      and division.
FAU - Dai, Zhi-kai
AU  - Dai ZK
AD  - Department of Pharmacology, Guilin Medical College, Guilin 541004.
FAU - Yang, Xiao-sheng
AU  - Yang XS
FAU - Yu, Li-mei
AU  - Yu LM
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - China
TA  - Zhongguo Zhong Xi Yi Jie He Za Zhi
JT  - Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese 
      journal of integrated traditional and Western medicine
JID - 9211576
RN  - 0 (Plant Extracts)
RN  - U3P01618RT (Fluorouracil)
SB  - IM
MH  - Adenocarcinoma/pathology
MH  - Cell Differentiation/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*drug effects
MH  - Endometrial Neoplasms/pathology
MH  - Female
MH  - Fluorouracil/*pharmacology
MH  - Herb-Drug Interactions
MH  - Humans
MH  - Plant Extracts/*pharmacology
MH  - Rosa/chemistry
EDAT- 2011/09/14 06:00
MHDA- 2013/08/02 06:00
CRDT- 2011/09/14 06:00
PHST- 2011/09/14 06:00 [entrez]
PHST- 2011/09/14 06:00 [pubmed]
PHST- 2013/08/02 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Xi Yi Jie He Za Zhi. 2011 Aug;31(8):1108-12, 1117.