PMID- 21911060
OWN - NLM
STAT- MEDLINE
DCOM- 20120113
LR  - 20191210
IS  - 1879-0240 (Electronic)
IS  - 0965-1748 (Linking)
VI  - 41
IP  - 11
DP  - 2011 Nov
TI  - Cloning and characterization of a dronc homologue in the silkworm, Bombyx mori.
PG  - 909-21
LID - 10.1016/j.ibmb.2011.08.005 [doi]
AB  - We cloned and characterized a novel Bombyx mori homologue (bm-dronc) of 
      Drosophila melanogaster dronc (dm-dronc), which could encode a polypeptide of 438 
      amino acid residues. Bm-Dronc shares relatively low amino acid sequence 
      identities of 25% and 26% with Dm-Dronc and Aedes aegypti Dronc (Aa-Dronc), 
      respectively. Bm-Dronc has the sequence QACRG surrounding the catalytic site (C), 
      which is consistent with the QAC(R/Q/G)(G/E) consensus sequence in most caspases 
      but distinct from the sequences PFCRG and SICRG of Dm-Dronc and Aa-Dronc, 
      respectively. Bm-Dronc possesses a long N-terminal prodomain containing a caspase 
      recruitment domain (CARD), a p20 domain and a p10 domain, exhibiting cleavage 
      activities on synthetic substrates Ac-VDVAD-AMC, Ac-IETD-AMC and Ac-LEHD-AMC, 
      which are preferred by human initiator caspases-2, -8 and -9, respectively. 
      Bm-Dronc transiently expressed in insect cells and Escherichia coli cells 
      underwent spontaneous cleavage and caused apoptosis and stimulation of 
      caspase-3-like protease activity in various lepidopteran cell lines, but not in 
      the dipteran cell line D. melanogaster S2. The apoptosis and the stimulation of 
      caspase-3-like protease activity induced by Bm-Dronc overexpression were 
      abrogated upon transfection with either a double-stranded RNA against bm-dronc or 
      a plasmid expressing functional anti-apoptotic protein Hycu-IAP3 encoded by the 
      baculovirus Hyphantria cunea multiple nucleopolyhedrovirus (MNPV). Apoptosis 
      induction in BM-N cells by infection with a p35-defective Autographa californica 
      MNPV or exposure to actinomycin D and UV promoted the cleavage of Bm-Dronc. These 
      results indicate that Bm-Dronc serves as the initiator caspase responsible for 
      the induction of caspase-dependent apoptosis.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Suganuma, Ikue
AU  - Suganuma I
AD  - Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 
      464-8601, Japan.
FAU - Ushiyama, Takayoshi
AU  - Ushiyama T
FAU - Yamada, Hayato
AU  - Yamada H
FAU - Iwamoto, Asako
AU  - Iwamoto A
FAU - Kobayashi, Michihiro
AU  - Kobayashi M
FAU - Ikeda, Motoko
AU  - Ikeda M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110908
PL  - England
TA  - Insect Biochem Mol Biol
JT  - Insect biochemistry and molecular biology
JID - 9207282
RN  - 0 (Drosophila Proteins)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - EC 3.4.22.- (Caspases)
RN  - EC 3.4.22.- (dronc protein, Drosophila)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apoptosis
MH  - Bombyx/*enzymology
MH  - Caspases/*chemistry
MH  - Cell Line
MH  - Dactinomycin
MH  - Drosophila Proteins/*chemistry
MH  - Escherichia coli
MH  - Gene Expression Regulation
MH  - Molecular Sequence Data
MH  - Nucleopolyhedroviruses
MH  - Sequence Analysis, DNA
MH  - Substrate Specificity
MH  - Ultraviolet Rays
EDAT- 2011/09/14 06:00
MHDA- 2012/01/14 06:00
CRDT- 2011/09/14 06:00
PHST- 2011/07/26 00:00 [received]
PHST- 2011/08/25 00:00 [revised]
PHST- 2011/08/29 00:00 [accepted]
PHST- 2011/09/14 06:00 [entrez]
PHST- 2011/09/14 06:00 [pubmed]
PHST- 2012/01/14 06:00 [medline]
AID - S0965-1748(11)00163-9 [pii]
AID - 10.1016/j.ibmb.2011.08.005 [doi]
PST - ppublish
SO  - Insect Biochem Mol Biol. 2011 Nov;41(11):909-21. doi: 10.1016/j.ibmb.2011.08.005. 
      Epub 2011 Sep 8.