PMID- 21911062
OWN - NLM
STAT- MEDLINE
DCOM- 20120202
LR  - 20131121
IS  - 1878-5492 (Electronic)
IS  - 0966-3274 (Linking)
VI  - 25
IP  - 4
DP  - 2011 Dec
TI  - Effects of chimerism on the mice heart transplanted survival with the bone marrow 
      infusion.
PG  - 202-6
LID - 10.1016/j.trim.2011.08.006 [doi]
AB  - AIMS: To evaluate the effects of chimerism on the mice heart transplanted 
      survival with the bone marrow infusion. METHODS: Bone marrow cells (BMCs) were 
      obtained from BALB/c mice. These BMCs were injected into the irradiated 
      (2Gy-Co60) C57BL/6 mice through femoral vein. Then Group A mice were treated with 
      Cyclosporine (1mg/kg) for 21days and Group B were not treated with Cyclosporine. 
      Group C were treated as the control group without BMCs infusion. Group D were 
      treated with Cyclosporine (1mg/kg) for 21days pre-hearttransplantation without 
      BMCs infusion. After 21days, the C57BL/6 mice received heart allografts from 
      BALB/c. To determine the degree of chimerism in BMCs infusion recipients, 
      peripheral blood were isolated on day 7, 14, 21. Allografts were harvested 10days 
      after heart transplantation for the histological analysis. RESULTS: (1) Chimerism 
      detected in the peripheral blood of Group A mice on day 7 after BMCs infusion was 
      6.1+/-2.5%, on day 14 was 15.4+/-2.9% and on day 21 was 10.7+/-2.6%. For the Group B 
      mice on day 7 after BMCs infusion, the chimerism was 2.8+/-1.1%, on day 14 was 
      11.2+/-4.8% and on day 21 was 7.4+/-3.7%. For the Groups C and D mice, no chimerism 
      was observed. Group A mice had the tendency toward improved level of chimerism 
      than Group B mice. (2) The survival time of Group A (n=6) was 13.0+/-1.4days which 
      was significantly longer than Group B (n=6) with the survival time was 
      8.5+/-1.3days (p<0.001), also longer than the mice in Groups C and D, the survival 
      time of which were 10.0+/-1.3days (p=0.008) and 9.4+/-1.1days (p=0.004). There is no 
      significant difference among Groups B, C, and D. (3) The HE staining showed the 
      much more seriously heart rejection in Groups B, C and D than Group A. 
      CONCLUSIONS: The chimerism was found in the BMCs infusion groups. Without the CsA 
      treatment combined with chimerism could not protect the transplanted heart. There 
      was no obvious evidence showed that the chimerism alone could improve the 
      survival time of cardiac allografts in mice.
CI  - Published by Elsevier B.V.
FAU - Jiang, Hong
AU  - Jiang H
AD  - Kidney Disease Center, The First Affiliated Hospital, College of Medicine, 
      Zhejiang University, 310003, PR China.
FAU - Tu, Haiyan
AU  - Tu H
FAU - Chen, Zhimin
AU  - Chen Z
FAU - Chen, Rongjun
AU  - Chen R
FAU - Wang, Yucheng
AU  - Wang Y
FAU - Wang, Minmin
AU  - Wang M
FAU - Jin, Juan
AU  - Jin J
FAU - Feng, Shi
AU  - Feng S
FAU - Chen, Wenqing
AU  - Chen W
FAU - Bi, Yan
AU  - Bi Y
FAU - Wang, Huiping
AU  - Wang H
FAU - Mao, Youying
AU  - Mao Y
FAU - Shou, Zhangfei
AU  - Shou Z
FAU - Chen, Jianghua
AU  - Chen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110902
PL  - Netherlands
TA  - Transpl Immunol
JT  - Transplant immunology
JID - 9309923
RN  - 0 (Immunosuppressive Agents)
RN  - 83HN0GTJ6D (Cyclosporine)
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation/*immunology
MH  - Cyclosporine/pharmacology
MH  - Graft Survival/drug effects/*immunology
MH  - *Heart
MH  - Heart Transplantation/*immunology
MH  - Immunosuppressive Agents/pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Time Factors
MH  - Transplantation Chimera/*immunology
MH  - Transplantation, Homologous
EDAT- 2011/09/14 06:00
MHDA- 2012/02/03 06:00
CRDT- 2011/09/14 06:00
PHST- 2011/03/11 00:00 [received]
PHST- 2011/08/07 00:00 [revised]
PHST- 2011/08/20 00:00 [accepted]
PHST- 2011/09/14 06:00 [entrez]
PHST- 2011/09/14 06:00 [pubmed]
PHST- 2012/02/03 06:00 [medline]
AID - S0966-3274(11)00096-7 [pii]
AID - 10.1016/j.trim.2011.08.006 [doi]
PST - ppublish
SO  - Transpl Immunol. 2011 Dec;25(4):202-6. doi: 10.1016/j.trim.2011.08.006. Epub 2011 
      Sep 2.