PMID- 21911137
OWN - NLM
STAT- MEDLINE
DCOM- 20120110
LR  - 20110913
IS  - 1873-2623 (Electronic)
IS  - 0041-1345 (Linking)
VI  - 43
IP  - 7
DP  - 2011 Sep
TI  - Monitoring of cytomegalovirus-specific CD8+ T-cell response with major 
      histocompatibility complex pentamers in kidney transplant recipients.
PG  - 2636-40
LID - 10.1016/j.transproceed.2011.05.051 [doi]
AB  - INTRODUCTION: Cytomegalovirus (CMV) can reactivate causing serious clinical 
      problems during immunosuppression. CMV-specific CD8(+) T cells play an important 
      role in the control of CMV reactivation. Using pentameric major 
      histocompatibility complex (MHC) peptide complexes, we investigated cellular 
      immune responses to CMV among healthy individuals and kidney transplantation 
      recipients in Korea, which is an endemic area of CMV infection. MATERIALS AND 
      METHODS: Analysis of CMV-specific T cells was performed on 28 healthy individuals 
      and 40 recipients who bore human leukocyte antigen (HLA)-A2 or -A24. CMV pp65 
      pentamer-binding cells incubated with various monoclonal antibodies were measured 
      by four-color flow cytometry. RESULTS: Detectable levels of pentamer(+) CD8(+) T 
      cells were present in 109/139 samples (78.4%) that stained with the 
      A*02NLV-pentamer, while 15/67 samples (22.4%) stained with the A*24QYD-pentamer 
      (P < .01). Among patients with HLA-A2, 22/24 (91.7%) samples showing positive CMV 
      antigenemia revealed detectable pentamer(+) CD8(+) T cells, while 87/115 (75.7%) 
      displaying negative CMV antigenemia had detectable pentamer(+) CD8(+) T cells (P 
      = .04). There was no significant difference in percentages of pentamer(+) CD8(+) 
      T cells between patients who did versus who did not experience episodes of CMV 
      infection. The subpopulation of CMV-specific CD8(+) T cells in transplantation 
      recipients was evaluated using phenotypic markers; memory cells comprised the 
      majority of the CMV-specific CD8(+) T-cell population. CONCLUSION: The 
      A*02NLV-pentamer complex was useful to monitor CMV-specific T cells. However, MHC 
      pentamer-based techniques did not provide a clear distinction between patients 
      who are or are not at risk for CMV infection.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Lee, S
AU  - Lee S
AD  - Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of 
      Medicine, Seoul, Korea.
FAU - Park, J B
AU  - Park JB
FAU - Kim, E Y
AU  - Kim EY
FAU - Joo, S Y
AU  - Joo SY
FAU - Shin, E C
AU  - Shin EC
FAU - Kwon, C H
AU  - Kwon CH
FAU - Joh, J W
AU  - Joh JW
FAU - Kim, S J
AU  - Kim SJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
SB  - IM
MH  - Adult
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Case-Control Studies
MH  - Cytomegalovirus/*immunology
MH  - Female
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - *Kidney Transplantation
MH  - *Major Histocompatibility Complex
MH  - Male
MH  - Middle Aged
MH  - Monitoring, Physiologic
EDAT- 2011/09/14 06:00
MHDA- 2012/01/11 06:00
CRDT- 2011/09/14 06:00
PHST- 2011/04/21 00:00 [received]
PHST- 2011/05/09 00:00 [accepted]
PHST- 2011/09/14 06:00 [entrez]
PHST- 2011/09/14 06:00 [pubmed]
PHST- 2012/01/11 06:00 [medline]
AID - S0041-1345(11)00866-9 [pii]
AID - 10.1016/j.transproceed.2011.05.051 [doi]
PST - ppublish
SO  - Transplant Proc. 2011 Sep;43(7):2636-40. doi: 10.1016/j.transproceed.2011.05.051.