PMID- 21911248
OWN - NLM
STAT- MEDLINE
DCOM- 20120611
LR  - 20131121
IS  - 1879-2472 (Electronic)
IS  - 0049-3848 (Linking)
VI  - 128
IP  - 6
DP  - 2011 Dec
TI  - In vitro recovery of Th1/Th2 balance in PBMCs from patients with immune 
      thrombocytopenia through the actions of IL-18BPa/Fc.
PG  - e119-24
LID - 10.1016/j.thromres.2011.07.012 [doi]
AB  - To determine the effects of IL-18BPa/Fc on the cytokine production and survival 
      of peripheral blood mononuclear cells (PBMCs) of immune thrombocytopenia (ITP), 
      PBMCs isolated from patients with ITP and healthy donors were treated with or 
      without of IL-18BPa/Fc. The production of IFN-gamma, IL-2, tumor necrosis factor 
      (TNF)-alpha, IL-4, IL-5 and IL-10 was measured by ELISA, and mRNA expression of IFN-gamma 
      and IL-18R was evaluated by RT-PCR. Besides, flow cytometric analysis of cell 
      apoptosis was performed by staining with annexin V-FITC/ Propidium Iodide (PI). 
      The proliferation rate of PBMCs was examined by CCK-8 assay. IL-18BPa/Fc at 10 
      ng/ml significantly stimulated IL-10 secretion from PBMCs in patients with ITP 
      and healthy donors, while it decreased IFN-gamma release. Further, IL-18BPa/Fc 
      enhanced dexamethason(DEX) reduction of PHA-induced IFN-gamma production by an 
      additional 38.9%(DEX 20 nmol/l) and 49.9%(DEX 50 nmol/l) in ITP patients. 
      Interestingly, the treatment of PBMCs with IL-18BPa/Fc increased the percentage 
      of early apoptotic cells in patients with ITP. In conclusion, IL-18BPa/Fc, via 
      neutralizing the biologic activity of mature IL-18, accelerates lymphocyte 
      apoptosis and downregulates IFN-gamma, while permitting the production of Th2 
      cytokine IL-10. These observations suggest a role of IL-18BPa/Fc in the recovery 
      of Th1/Th2 balance, as well as its therapeutic potential in the treatment of ITP.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Shan, Ning-Ning
AU  - Shan NN
AD  - Department of Haematology, Provincial Hospital Affiliated to Shandong University, 
      Jinan, 250021, China.
FAU - Ji, Xue-Bin
AU  - Ji XB
FAU - Wang, Xin
AU  - Wang X
FAU - Li, Ying
AU  - Li Y
FAU - Liu, Xin
AU  - Liu X
FAU - Zhu, Xiao-Juan
AU  - Zhu XJ
FAU - Hou, Ming
AU  - Hou M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110910
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulin Fragments)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukin-18)
RN  - 0 (interleukin-18 binding protein)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Apoptosis/drug effects/immunology
MH  - Cell Growth Processes/drug effects/immunology
MH  - Cytokines/biosynthesis/blood/*immunology
MH  - Dexamethasone/pharmacology
MH  - Drug Synergism
MH  - Female
MH  - Humans
MH  - Immunoglobulin Fragments/immunology/*pharmacology
MH  - Intercellular Signaling Peptides and Proteins/immunology
MH  - Interleukin-18/immunology
MH  - Male
MH  - Middle Aged
MH  - Purpura, Thrombocytopenic, Idiopathic/blood/*immunology/*therapy
MH  - Th1 Cells/*drug effects/immunology
MH  - Th2 Cells/*drug effects/immunology
MH  - Young Adult
EDAT- 2011/09/14 06:00
MHDA- 2012/06/12 06:00
CRDT- 2011/09/14 06:00
PHST- 2011/02/15 00:00 [received]
PHST- 2011/06/02 00:00 [revised]
PHST- 2011/07/11 00:00 [accepted]
PHST- 2011/09/14 06:00 [entrez]
PHST- 2011/09/14 06:00 [pubmed]
PHST- 2012/06/12 06:00 [medline]
AID - S0049-3848(11)00358-6 [pii]
AID - 10.1016/j.thromres.2011.07.012 [doi]
PST - ppublish
SO  - Thromb Res. 2011 Dec;128(6):e119-24. doi: 10.1016/j.thromres.2011.07.012. Epub 
      2011 Sep 10.