PMID- 21914809
OWN - NLM
STAT- MEDLINE
DCOM- 20120109
LR  - 20220321
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 286
IP  - 44
DP  - 2011 Nov 4
TI  - Allele-dependent processing pathways generate the endogenous human leukocyte 
      antigen (HLA) class I peptide repertoire in transporters associated with antigen 
      processing (TAP)-deficient cells.
PG  - 38054-38059
LID - S0021-9258(20)50647-9 [pii]
LID - 10.1074/jbc.M111.281808 [doi]
AB  - The transporters associated with antigen processing (TAP) allow the supply of 
      peptides derived from the cytosol to translocate to the endoplasmic reticulum, 
      where they complex with nascent human leukocyte antigen (HLA) class I molecules. 
      However, infected and tumor cells with TAP molecules blocked or individuals with 
      nonfunctional TAP complexes are able to present HLA class I ligands generated by 
      TAP-independent processing pathways. These peptides are detected by the CD8(+) 
      lymphocyte cellular response. Here, the generation of the overall peptide 
      repertoire associated with four different HLA class I molecules in TAP-deficient 
      cells was studied. Using different protease inhibitors, four different 
      proteolytic specificities were identified. These data demonstrate the different 
      allele-dependent complex processing pathways involved in the generation of the 
      HLA class I peptide repertoire in TAP-deficient cells.
FAU - Lorente, Elena
AU  - Lorente E
AD  - Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 
      Majadahonda (Madrid), Spain.
FAU - Garcia, Ruth
AU  - Garcia R
AD  - Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 
      Majadahonda (Madrid), Spain.
FAU - Lopez, Daniel
AU  - Lopez D
AD  - Centro Nacional de Microbiologia, Instituto de Salud Carlos III, 28220 
      Majadahonda (Madrid), Spain. Electronic address: dlopez@isciii.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110913
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 2)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Antigens)
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Ligands)
RN  - 0 (Peptides)
RN  - 0 (Protease Inhibitors)
RN  - 0 (TAP1 protein, human)
RN  - EC 3.4.- (Peptide Hydrolases)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 2
MH  - ATP-Binding Cassette Transporters/*metabolism
MH  - Alleles
MH  - Antigens/chemistry
MH  - CD8-Positive T-Lymphocytes/cytology/metabolism
MH  - Cell Line
MH  - Cytosol/metabolism
MH  - Endoplasmic Reticulum/metabolism
MH  - HLA Antigens/*chemistry
MH  - Histocompatibility Antigens Class I/*chemistry
MH  - Humans
MH  - Ligands
MH  - Peptide Hydrolases/chemistry
MH  - Peptides/chemistry
MH  - Protease Inhibitors/pharmacology
MH  - Protein Transport
MH  - Surface Properties
PMC - PMC3207402
EDAT- 2011/09/15 06:00
MHDA- 2012/01/10 06:00
PMCR- 2012/11/04
CRDT- 2011/09/15 06:00
PHST- 2011/09/15 06:00 [entrez]
PHST- 2011/09/15 06:00 [pubmed]
PHST- 2012/01/10 06:00 [medline]
PHST- 2012/11/04 00:00 [pmc-release]
AID - S0021-9258(20)50647-9 [pii]
AID - M111.281808 [pii]
AID - 10.1074/jbc.M111.281808 [doi]
PST - ppublish
SO  - J Biol Chem. 2011 Nov 4;286(44):38054-38059. doi: 10.1074/jbc.M111.281808. Epub 
      2011 Sep 13.