PMID- 21917091
OWN - NLM
STAT- MEDLINE
DCOM- 20120604
LR  - 20111110
IS  - 1600-0854 (Electronic)
IS  - 1398-9219 (Linking)
VI  - 12
IP  - 12
DP  - 2011 Dec
TI  - Dynamic imaging of cell-free and cell-associated viral capture in mature 
      dendritic cells.
PG  - 1702-13
LID - 10.1111/j.1600-0854.2011.01281.x [doi]
AB  - Dendritic cells (DCs) capture human immunodeficiency virus (HIV) through a 
      non-fusogenic mechanism that enables viral transmission to CD4(+) T cells, 
      contributing to in vivo viral dissemination. Although previous studies have 
      provided important clues to cell-free viral capture by mature DCs (mDCs), dynamic 
      and kinetic insight on this process is still missing. Here, we used 
      three-dimensional video microscopy and single-particle tracking approaches to 
      dynamically dissect both cell-free and cell-associated viral capture by living 
      mDCs. We show that cell-free virus capture by mDCs operates through three 
      sequential phases: virus binding through specific determinants expressed in the 
      viral particle, polarized or directional movements toward concrete regions of the 
      cell membrane and virus accumulation in a sac-like structure where trapped viral 
      particles display a hindered diffusive behavior. Moreover, real-time imaging of 
      cell-associated viral transfer to mDCs showed a similar dynamics to that 
      exhibited by cell-free virus endocytosis leading to viral accumulation in 
      compartments. However, cell-associated HIV type 1 transfer to mDCs was the most 
      effective pathway, boosted throughout enhanced cellular contacts with infected 
      CD4(+) T cells. Our results suggest that in lymphoid tissues, mDC viral uptake 
      could occur either by encountering cell-free or cell-associated virus produced by 
      infected cells generating the perfect scenario to promote HIV pathogenesis and 
      impact disease progression.
CI  - (c) 2011 John Wiley & Sons A/S.
FAU - Izquierdo-Useros, Nuria
AU  - Izquierdo-Useros N
AD  - AIDS Research Institute IrsiCaixa, Institut d'Investigacio en Ciencies de la 
      Salut Germans Trias i Pujol, Universitat Autonoma de Barcelona, 08916 Badalona, 
      Spain.
FAU - Esteban, Olga
AU  - Esteban O
FAU - Rodriguez-Plata, Maria T
AU  - Rodriguez-Plata MT
FAU - Erkizia, Itziar
AU  - Erkizia I
FAU - Prado, Julia G
AU  - Prado JG
FAU - Blanco, Julia
AU  - Blanco J
FAU - Garcia-Parajo, Maria F
AU  - Garcia-Parajo MF
FAU - Martinez-Picado, Javier
AU  - Martinez-Picado J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20111010
PL  - England
TA  - Traffic
JT  - Traffic (Copenhagen, Denmark)
JID - 100939340
SB  - IM
MH  - Biological Transport
MH  - CD4-Positive T-Lymphocytes/immunology/virology
MH  - Cell Membrane/immunology/virology
MH  - Cell-Free System
MH  - Cells, Cultured
MH  - Cytoskeleton/immunology/virology
MH  - Dendritic Cells/*immunology/*virology
MH  - Endocytosis/immunology
MH  - HIV Infections/*immunology/*virology
MH  - HIV-1/*immunology
MH  - Humans
MH  - Imaging, Three-Dimensional/methods
MH  - Microscopy, Video/methods
MH  - Virion/*immunology
MH  - Virus Attachment
EDAT- 2011/09/16 06:00
MHDA- 2012/06/05 06:00
CRDT- 2011/09/16 06:00
PHST- 2011/09/16 06:00 [entrez]
PHST- 2011/09/16 06:00 [pubmed]
PHST- 2012/06/05 06:00 [medline]
AID - 10.1111/j.1600-0854.2011.01281.x [doi]
PST - ppublish
SO  - Traffic. 2011 Dec;12(12):1702-13. doi: 10.1111/j.1600-0854.2011.01281.x. Epub 
      2011 Oct 10.