PMID- 21918012
OWN - NLM
STAT- MEDLINE
DCOM- 20120112
LR  - 20200228
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 92
IP  - Pt 12
DP  - 2011 Dec
TI  - New insights into RNA packaging in porcine reproductive and respiratory syndrome 
      virus.
PG  - 2865-2870
LID - 10.1099/vir.0.036079-0 [doi]
AB  - While identifying whether the smallest packaged heteroclite subgenomic RNA (S9) 
      of porcine reproductive and respiratory syndrome virus (PRRSV) contains a 
      packaging signal, we found that S9 was capable of binding to the basic amino 
      acid-rich domain (synthetic peptide of aa 34-53) of the packaging protein (N). In 
      addition, by using truncations at the 5' and 3' ends of S9, a minimal binding 
      region of 35 nt was found to be essential for binding to both the synthetic 
      peptide and to the full-length N protein. Furthermore, by using cell-culture 
      experiments, we found that S9 was capable of packaging non-viral RNA sequence 
      into PRRSV particles and that the 35 nt region was essential for this activity. 
      Taken together, our data suggest that this 35 nt region might be an important 
      element for packaging PRRSV genomic RNA into virus particles.
FAU - Baig, Tayyba T
AU  - Baig TT
AD  - Vaccine and Infectious Disease Organization-International Vaccine Centre 
      (VIDO-InterVac), 120 Veterinary Road, University of Saskatchewan, Saskatoon, SK 
      S7N 5E3, Canada.
FAU - Zakhartchouk, Alexander
AU  - Zakhartchouk A
AD  - Vaccine and Infectious Disease Organization-International Vaccine Centre 
      (VIDO-InterVac), 120 Veterinary Road, University of Saskatchewan, Saskatoon, SK 
      S7N 5E3, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110914
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (3' Untranslated Regions)
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Genetic Markers)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - 3' Untranslated Regions
MH  - 5' Untranslated Regions
MH  - Animals
MH  - Cells, Cultured
MH  - Genetic Markers
MH  - Porcine respiratory and reproductive syndrome 
      virus/*genetics/metabolism/*physiology
MH  - Protein Binding
MH  - RNA, Viral/genetics/metabolism
MH  - Sequence Analysis, RNA/methods
MH  - Swine/*virology
MH  - *Virus Assembly
EDAT- 2011/09/16 06:00
MHDA- 2012/01/13 06:00
CRDT- 2011/09/16 06:00
PHST- 2011/09/16 06:00 [entrez]
PHST- 2011/09/16 06:00 [pubmed]
PHST- 2012/01/13 06:00 [medline]
AID - 10.1099/vir.0.036079-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2011 Dec;92(Pt 12):2865-2870. doi: 10.1099/vir.0.036079-0. Epub 2011 
      Sep 14.