PMID- 21918017
OWN - NLM
STAT- MEDLINE
DCOM- 20120222
LR  - 20220311
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 49
IP  - 11
DP  - 2011 Nov
TI  - Rapid virological diagnosis of central nervous system infections by use of a 
      multiplex reverse transcription-PCR DNA microarray.
PG  - 3874-9
LID - 10.1128/JCM.01214-11 [doi]
AB  - Viruses are the main etiological cause of central nervous system (CNS) 
      infections. A rapid molecular diagnosis is recommended to improve the therapeutic 
      management of patients. The aim of this study was to evaluate the performances of 
      a DNA microarray, the Clart Entherpex kit (Genomica, Coslada, Spain), allowing 
      the rapid and simultaneous detection of 9 DNA and RNA neurotropic viruses: herpes 
      simplex virus 1 (HSV-1), HSV-2, varicella-zoster virus (VZV), cytomegalovirus 
      (CMV), Epstein-Barr virus (EBV), human herpesvirus 6 (HHV-6), HHV-7, HHV-8, and 
      the human enteroviruses (HEVs). This evaluation was performed with 28 samples 
      from the European proficiency panels (Quality Control for Molecular Diagnostics 
      [QCMD]; Glasgow, Scotland) and then with 78 cerebrospinal fluid (CSF) specimens. 
      The majority of the QCMD results obtained by the DNA microarray were similar to 
      those recorded by the overall QCMD participants. The main discrepant results were 
      observed for low concentrations of HSV-2 and HEVs. From the clinical samples, the 
      kit detected 27 of the 28 herpesvirus CNS infections and all of the 30 
      HEV-positive CSF samples. No false-positive result was observed among the 20 
      virus-negative CSF samples. The clinical sensitivity, specificity, and negative 
      and positive predictive values of the assay were 98.3, 100, 95.2, and 100%, 
      respectively, when the results were compared to those of commercially available 
      PCR assays. Interestingly, HHV-7 was detected in 11 (37%) of the 30 HEV-positive 
      CSF samples from children suffering from aseptic meningitis causing significantly 
      longer lengths of stay at the hospital than infection with HEVs alone (2.4 versus 
      1.4 days; P = 0.038). In conclusion, this preliminary study showed that this DNA 
      microarray could be a valuable molecular diagnostic tool for single and mixed DNA 
      and RNA virus infections of the CNS.
FAU - Leveque, Nicolas
AU  - Leveque N
AD  - Clinical and Molecular Virology Unit, University Hospital, and EA-4303 Faculty of 
      Medicine, Reims, France. nleveque@chu-reims.fr
FAU - Van Haecke, Adrien
AU  - Van Haecke A
FAU - Renois, Fanny
AU  - Renois F
FAU - Boutolleau, David
AU  - Boutolleau D
FAU - Talmud, Deborah
AU  - Talmud D
FAU - Andreoletti, Laurent
AU  - Andreoletti L
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110914
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
SB  - IM
MH  - Adult
MH  - Central Nervous System Infections/*diagnosis/*virology
MH  - Child
MH  - Child, Preschool
MH  - DNA Viruses/classification/isolation & purification
MH  - Female
MH  - Humans
MH  - Infant
MH  - Male
MH  - Molecular Diagnostic Techniques/*methods
MH  - Oligonucleotide Array Sequence Analysis/*methods
MH  - Predictive Value of Tests
MH  - RNA Viruses/classification/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
MH  - Sensitivity and Specificity
MH  - Virus Diseases/*diagnosis/*virology
PMC - PMC3209095
EDAT- 2011/09/16 06:00
MHDA- 2012/02/23 06:00
PMCR- 2012/05/01
CRDT- 2011/09/16 06:00
PHST- 2011/09/16 06:00 [entrez]
PHST- 2011/09/16 06:00 [pubmed]
PHST- 2012/02/23 06:00 [medline]
PHST- 2012/05/01 00:00 [pmc-release]
AID - JCM.01214-11 [pii]
AID - 1214-11 [pii]
AID - 10.1128/JCM.01214-11 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2011 Nov;49(11):3874-9. doi: 10.1128/JCM.01214-11. Epub 2011 
      Sep 14.