PMID- 21918999
OWN - NLM
STAT- MEDLINE
DCOM- 20150626
LR  - 20141103
IS  - 1097-0010 (Electronic)
IS  - 0022-5142 (Linking)
VI  - 92
IP  - 3
DP  - 2012 Feb
TI  - Extraction and mass spectrometry identification of a major peach allergen Pru p 
      1.
PG  - 570-6
LID - 10.1002/jsfa.4609 [doi]
AB  - BACKGROUND: Peach allergy can be caused by the allergen Pru p 1. This occurs by 
      cross-reactivity with the homologous birch pollen allergen Bet v 1. However, the 
      direct identification of Pru p 1 as an immunoglobulin E (IgE)-binding protein 
      extracted from peach fruit has never been reported. RESULTS: Phosphate-buffered 
      saline (PBS) and phenol extractions were applied to solubilise the proteins from 
      peach peel and pulp, and IgE immunoblotting with sera of individual 
      peach-allergic patients was used to detect the potential allergens. Most of the 
      patients showed binding to an 18 kDa band in IgE immunoblotting performed with 
      the phenolic extracts of peach peel and pulp, but not when the PBS extracts were 
      used. Mass spectrometry of the 18 kDa spot excised from a two-dimensional 
      electrophoretic gel showed this protein to correspond to the peach allergen Pru p 
      1. CONCLUSION: Phenol extraction was necessary to detect by IgE immunoblotting a 
      major peach allergen, which showed very low extractability with PBS, indicating 
      the appropriateness of adopting different extraction procedures to identify plant 
      allergens. The 18 kDa peach protein was definitively identified as the Bet v 
      1-homologous peach allergen Pru p 1.
CI  - Copyright (c) 2011 Society of Chemical Industry.
FAU - Pasini, Gabriella
AU  - Pasini G
AD  - Dipartimento Biotecnologie Agrarie, Universita Padova, Viale dell'Universita 16, 
      I-35020 Legnaro (PD), Italy.
FAU - Curioni, Andrea
AU  - Curioni A
FAU - Vegro, Mara
AU  - Vegro M
FAU - Pagani, Mauro
AU  - Pagani M
FAU - Masi, Antonio
AU  - Masi A
FAU - Schievano, Elisabetta
AU  - Schievano E
FAU - Antico, Andrea
AU  - Antico A
LA  - eng
PT  - Journal Article
DEP - 20110914
PL  - England
TA  - J Sci Food Agric
JT  - Journal of the science of food and agriculture
JID - 0376334
RN  - 0 (Antigens, Plant)
RN  - 0 (Plant Proteins)
RN  - 0 (Pru p 1 allergen)
RN  - 0 (Solvents)
RN  - 339NCG44TV (Phenol)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Antibody Specificity
MH  - Antigens, Plant/adverse effects/*analysis/chemistry/isolation & purification
MH  - Chromatography, High Pressure Liquid
MH  - Female
MH  - Food Hypersensitivity/blood/complications/etiology/*immunology
MH  - Fruit/adverse effects/*chemistry
MH  - Humans
MH  - Immunoglobulin E/analysis/metabolism
MH  - Italy
MH  - Male
MH  - Middle Aged
MH  - Molecular Weight
MH  - Phenol/chemistry
MH  - Plant Proteins/adverse effects/*analysis/chemistry/isolation & purification
MH  - Prunus/adverse effects/*chemistry
MH  - Rhinitis, Allergic, Seasonal/blood/complications/etiology/*immunology
MH  - Solvents/chemistry
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Surface Properties
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Pru p 1
OT  - Pru p 3
OT  - electrophoresis
OT  - mass spectrometry
OT  - peach allergy
EDAT- 2011/09/16 06:00
MHDA- 2015/06/27 06:00
CRDT- 2011/09/16 06:00
PHST- 2010/10/04 00:00 [received]
PHST- 2011/07/19 00:00 [revised]
PHST- 2011/07/19 00:00 [accepted]
PHST- 2011/09/16 06:00 [entrez]
PHST- 2011/09/16 06:00 [pubmed]
PHST- 2015/06/27 06:00 [medline]
AID - 10.1002/jsfa.4609 [doi]
PST - ppublish
SO  - J Sci Food Agric. 2012 Feb;92(3):570-6. doi: 10.1002/jsfa.4609. Epub 2011 Sep 14.