PMID- 21920393
OWN - NLM
STAT- MEDLINE
DCOM- 20120504
LR  - 20120113
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 163
IP  - 1
DP  - 2012 Jan
TI  - Genetic and phenotypic characterization of a 2006 United States porcine 
      reproductive and respiratory virus isolate associated with high morbidity and 
      mortality in the field.
PG  - 98-107
LID - 10.1016/j.virusres.2011.08.017 [doi]
AB  - The objective of this study was to characterize a porcine reproductive and 
      respiratory syndrome virus (PRRSV) isolated from United States pigs experiencing 
      high morbidity (50%) and mortality (20%). The PRRSV isolate, designated NC16845b, 
      was characterized through phenotypic analysis and genomic sequencing and compared 
      to Type 2 PRRSV isolates VR-2332, MN184 and VR-2385. NC16845b demonstrated slower 
      replication in vitro compared to the three other isolates and grew to a peak 
      titer of 5.4x10(5) plaque forming units (PFU) per ml at 60 h post inoculation, 
      which was 4- to 13-fold less than the peak titer of the other three viruses. 
      NC16845b plaques were intermediate size averaging 3.3mm in diameter that was 
      larger than MN184 plaques and smaller than VR-2385 and VR-2332. Using Northern 
      blot analysis, viral and subgenomic RNA were detected that demonstrated variable 
      levels of hybridization in some open reading frames (ORF) compared to the other 
      viruses. NC16845b is 15,389 nucleotides in length and ORF 5 restriction fragment 
      length polymorphism (RFLP) analysis demonstrated a 1-18-2 pattern. Among all 
      available Type 2 complete genome sequences, NC16845b showed the highest 
      nucleotide homology (91.2%) to atypical PRRSV strain JA142. Compared to prototype 
      VR-2332, NC16845b demonstrated marked nucleotide variability within 
      non-structural protein (nsp) 1beta and nsp2, and a nucleotide deletion of 24 bases 
      in nsp2. Sequence homology with VR-2332 and MN184 was 88.4% and 82.9%, 
      respectively; homology with the ORF2-7 of VR-2385 was 90.4%. Collectively, these 
      data indicate that, compared to prototype Type 2 PRRSV isolates, NC16845b 
      exhibited slower in vitro growth properties, had regions of heterogeneity within 
      ORF1a that corresponded to at least two individual virus quasispecies, and also 
      contained a continuous 8 amino acid deletion in the nsp2 protein.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Gauger, Phillip C
AU  - Gauger PC
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
FAU - Faaberg, Kay S
AU  - Faaberg KS
FAU - Guo, Baoqing
AU  - Guo B
FAU - Kappes, Matthew A
AU  - Kappes MA
FAU - Opriessnig, Tanja
AU  - Opriessnig T
LA  - eng
SI  - GENBANK/HQ699067
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110908
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cluster Analysis
MH  - Genome, Viral
MH  - Molecular Sequence Data
MH  - Nucleic Acid Hybridization
MH  - Phylogeny
MH  - Polymorphism, Genetic
MH  - Polymorphism, Restriction Fragment Length
MH  - Porcine Reproductive and Respiratory Syndrome/mortality/*pathology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/isolation & 
      purification/*pathogenicity
MH  - RNA, Viral/genetics/isolation & purification
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
MH  - Swine
MH  - United States
MH  - Viral Load
MH  - Viral Plaque Assay
MH  - Virus Replication
EDAT- 2011/09/17 06:00
MHDA- 2012/05/05 06:00
CRDT- 2011/09/17 06:00
PHST- 2011/02/08 00:00 [received]
PHST- 2011/08/26 00:00 [revised]
PHST- 2011/08/30 00:00 [accepted]
PHST- 2011/09/17 06:00 [entrez]
PHST- 2011/09/17 06:00 [pubmed]
PHST- 2012/05/05 06:00 [medline]
AID - S0168-1702(11)00323-6 [pii]
AID - 10.1016/j.virusres.2011.08.017 [doi]
PST - ppublish
SO  - Virus Res. 2012 Jan;163(1):98-107. doi: 10.1016/j.virusres.2011.08.017. Epub 2011 
      Sep 8.