PMID- 21922275
OWN - NLM
STAT- MEDLINE
DCOM- 20120430
LR  - 20211020
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 33
IP  - 2
DP  - 2012 Apr
TI  - Promoter methylation status and expression of estrogen receptor alpha in familial 
      breast cancer patients.
PG  - 413-20
LID - 10.1007/s13277-011-0234-x [doi]
AB  - The hypermethylation of estrogen receptor alpha (ERalpha) promoter is a common 
      molecular alteration in sporadic breast cancer (BC), but its involvement in 
      familial BC remains largely unknown. In the present study, we analyzed the 
      methylation statuses of four regions (ER1, ER3, ER4, and ER5) of the ERalpha promoter 
      and the ERalpha expression levels of 113 familial BC patients in a Han Chinese 
      Population from northeastern China and evaluated the association between major 
      clinicopathological features and the hypermethylation statuses of the ERalpha gene. 
      Tumor samples were analyzed for ERalpha methylation status by the 
      methylation-specific polymerase chain reaction for ERalpha, PR, p53, BRCA-1, and 
      BRCA-2 by immunohistochemical (IHC) staining and for Her-2 status by IHC and 
      fluorescence in situ hybridization (FISH). ERalpha methylation was observed in tumor 
      tissues in 47/113 (41.6%) familial BC patients. There were no significant 
      differences in the methylation statuses among ER1 (20.4%), ER3 (18.6%), ER4 
      (17.7%), and ER5 (19.5%; chi (2) = 3.89, p > 0.05). An association between ERalpha 
      expression level and its promoter methylation level was found. In addition, ERalpha 
      methylation was significantly correlated with tumor size, PR expression, p53 
      nuclear accumulation, and BRCA-1 and BRCA-2 statuses. In conclusion, in familial 
      BC patients, the level of ERalpha gene promoter methylation correlates with ERalpha 
      expression, PR, p53 nuclear accumulation, and BRCA-1 and BRCA-2 statuses. 
      Epigenetic alteration of ERalpha gene may play an important role in the pathogenesis 
      of familial BC.
FAU - Wei, Jing
AU  - Wei J
AD  - Departments of Breast Surgery and Surgical Oncology, Research Unit of General 
      Surgery, The First Affiliated Hospital of China Medical University, Shenyang, 
      Liaoning Province, 110001, China.
FAU - Han, Bing
AU  - Han B
FAU - Mao, Xiao-yun
AU  - Mao XY
FAU - Wei, Min-jie
AU  - Wei MJ
FAU - Yao, Fan
AU  - Yao F
FAU - Jin, Feng
AU  - Jin F
LA  - eng
PT  - Journal Article
DEP - 20110916
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - 0 (ESR1 protein, human)
RN  - 0 (Estrogen Receptor alpha)
SB  - IM
MH  - Adult
MH  - Breast Neoplasms/ethnology/*genetics
MH  - China
MH  - *DNA Methylation
MH  - Estrogen Receptor alpha/*genetics
MH  - Family Health
MH  - Female
MH  - Gene Expression Profiling/methods
MH  - *Gene Expression Regulation, Neoplastic
MH  - Genes, BRCA1
MH  - Genes, BRCA2
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - *Promoter Regions, Genetic
EDAT- 2011/09/17 06:00
MHDA- 2012/05/01 06:00
CRDT- 2011/09/17 06:00
PHST- 2011/07/12 00:00 [received]
PHST- 2011/08/26 00:00 [accepted]
PHST- 2011/09/17 06:00 [entrez]
PHST- 2011/09/17 06:00 [pubmed]
PHST- 2012/05/01 06:00 [medline]
AID - 10.1007/s13277-011-0234-x [doi]
PST - ppublish
SO  - Tumour Biol. 2012 Apr;33(2):413-20. doi: 10.1007/s13277-011-0234-x. Epub 2011 Sep 
      16.