PMID- 21922383
OWN - NLM
STAT- MEDLINE
DCOM- 20111230
LR  - 20211020
IS  - 1860-1499 (Electronic)
IS  - 1860-1499 (Linking)
VI  - 44
IP  - 3
DP  - 2011 Sep
TI  - Effects of short-term estrogen treatment on the progression of 
      N-methyl-N-nitrosourea-induced premalignant mammary lesions in female Lewis rats.
PG  - 125-30
LID - 10.1007/s00795-010-0515-2 [doi]
AB  - We studied the effects of short-term estrogen treatment (STET) on the progression 
      of mammary lesions from ductal hyperplasia (DH) through ductal carcinoma in situ 
      (DCIS) to invasive ductal carcinoma (IDC) in the N-methyl-N-nitrosourea 
      (MNU)-induced rat mammary carcinogenesis model. Three-week-old female Lewis rats 
      (n = 40) received an intraperitoneal injection of MNU (50 mg/kg). Three weeks 
      later, a 3-week-release, 0.25-mg, 17beta-estradiol pellet was subcutaneously 
      implanted for 2 weeks in 20 rats (STET); the remaining 20 rats did not receive 
      the estradiol pellets (age-matched control). All rats were killed at 12 weeks of 
      age, and their abdominal-inguinal mammary glands were histologically examined. 
      The incidence and multiplicity of DHs were similar between groups (STET, 90% and 
      3.9 +/- 0.6 vs. age-matched controls, 80% and 3.0 +/- 0.5). However, DCIS and IDC did 
      not develop in STET rats, whereas DCIS (25% and 1.4 +/- 0.2) and IDC (35% and 1.4 +/- 
      0.3) developed in the age-matched controls. Immunoscores of estrogen and 
      progesterone receptors and positive rate of proliferative cell nuclear antigen 
      (PCNA) in DH were similar in both groups, while the positive rate of cyclin D1 
      was significantly reduced in the STET group (P < 0.05). Thus, STET blocked the 
      progression from DH to DCIS in MNU-induced mammary carcinogenesis, and decreased 
      expression of cyclin D1 may play an important role in the blockade of cell 
      transition from DH to DCIS.
FAU - Yuri, Takashi
AU  - Yuri T
AD  - Department of Pathology II, Kansai Medical University, Moriguchi, Osaka 570-8506, 
      Japan. yurit@takii.kmu.ac.jp
FAU - Lai, Yen-Chang
AU  - Lai YC
FAU - Kanematsu, Sayaka
AU  - Kanematsu S
FAU - Kuwata, Maki
AU  - Kuwata M
FAU - Yoshizawa, Katsuhiko
AU  - Yoshizawa K
FAU - Tsubura, Airo
AU  - Tsubura A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110916
PL  - Japan
TA  - Med Mol Morphol
JT  - Medical molecular morphology
JID - 101239023
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Ccnd1 protein, rat)
RN  - 0 (Estrogens)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 136601-57-5 (Cyclin D1)
RN  - 684-93-5 (Methylnitrosourea)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/pharmacology/*therapeutic use
MH  - Cell Transformation, Neoplastic/*drug effects
MH  - Cyclin D1/metabolism
MH  - Estrogens/pharmacology/*therapeutic use
MH  - Female
MH  - Mammary Glands, Animal/drug effects/metabolism/pathology
MH  - Mammary Neoplasms, Experimental/*chemically induced/*drug therapy/pathology
MH  - Methylnitrosourea
MH  - Precancerous Conditions/*chemically induced/*drug therapy/pathology
MH  - Proliferating Cell Nuclear Antigen/metabolism
MH  - Rats
MH  - Rats, Inbred Lew
EDAT- 2011/09/17 06:00
MHDA- 2011/12/31 06:00
CRDT- 2011/09/17 06:00
PHST- 2010/02/10 00:00 [received]
PHST- 2010/05/14 00:00 [accepted]
PHST- 2011/09/17 06:00 [entrez]
PHST- 2011/09/17 06:00 [pubmed]
PHST- 2011/12/31 06:00 [medline]
AID - 10.1007/s00795-010-0515-2 [doi]
PST - ppublish
SO  - Med Mol Morphol. 2011 Sep;44(3):125-30. doi: 10.1007/s00795-010-0515-2. Epub 2011 
      Sep 16.