PMID- 21922433
OWN - NLM
STAT- MEDLINE
DCOM- 20120112
LR  - 20211020
IS  - 1869-1889 (Electronic)
IS  - 1674-7305 (Print)
IS  - 1674-7305 (Linking)
VI  - 54
IP  - 9
DP  - 2011 Sep
TI  - Mutational analysis of the SDD sequence motif of a PRRSV RNA-dependent RNA 
      polymerase.
PG  - 870-9
LID - 10.1007/s11427-011-4216-4 [doi]
AB  - The subgenomic mRNA transcription and genomic replication of the porcine 
      reproductive and respiratory syndrome virus (PRRSV) are directed by the viral 
      replicase. The replicase is expressed in the form of two polyproteins and is 
      subsequently processed into smaller nonstructural proteins (nsps). nsp9, 
      containing the viral replicase, has characteristic sequence motifs conserved 
      among the RNA-dependent RNA polymerases (RdRp) of positive-strand (PS) RNA 
      viruses. To test whether the conserved SDD motif can tolerate other conserved 
      motifs of RNA viruses and the influence of every residue on RdRp catalytic 
      activity, many amino acids substitutions were introduced into it. Only one nsp9 
      substitution, of serine by glycine (S3050G), could rescue mutant viruses. The 
      rescued virus was genetically stable. Alteration of either aspartate residue was 
      not tolerated, destroyed the polymerase activity, and abolished virus 
      transcription, but did not eliminate virus replication. We also found that the 
      SDD motif was essentially invariant for the signature sequence of PRRSV RdRp. It 
      could not accommodate other conserved motifs found in other RNA viral 
      polymerases, except the GDD motif, which is conserved in all the other PS RNA 
      viruses. These findings indicated that nidoviruses are evolutionarily related to 
      other PS RNA viruses. Our studies support the idea that the two aspartate 
      residues of the SDD motif are critical and essential for PRRSV transcription and 
      represent a sequence variant of the GDD motif in PS RNA viruses.
FAU - Zhou, Yan
AU  - Zhou Y
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, China.
FAU - Zheng, Haihong
AU  - Zheng H
FAU - Gao, Fei
AU  - Gao F
FAU - Tian, Debin
AU  - Tian D
FAU - Yuan, Shishan
AU  - Yuan S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110916
PL  - China
TA  - Sci China Life Sci
JT  - Science China. Life sciences
JID - 101529880
RN  - EC 2.7.7.48 (RNA-Dependent RNA Polymerase)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Humans
MH  - *Mutation
MH  - Plasmids
MH  - Polymerase Chain Reaction
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - RNA-Dependent RNA Polymerase/*metabolism
PMC - PMC7088696
EDAT- 2011/09/17 06:00
MHDA- 2012/01/13 06:00
PMCR- 2011/09/16
CRDT- 2011/09/17 06:00
PHST- 2011/05/22 00:00 [received]
PHST- 2011/07/03 00:00 [accepted]
PHST- 2011/09/17 06:00 [entrez]
PHST- 2011/09/17 06:00 [pubmed]
PHST- 2012/01/13 06:00 [medline]
PHST- 2011/09/16 00:00 [pmc-release]
AID - 4216 [pii]
AID - 10.1007/s11427-011-4216-4 [doi]
PST - ppublish
SO  - Sci China Life Sci. 2011 Sep;54(9):870-9. doi: 10.1007/s11427-011-4216-4. Epub 
      2011 Sep 16.