PMID- 21924167
OWN - NLM
STAT- MEDLINE
DCOM- 20120105
LR  - 20110919
IS  - 0091-679X (Print)
IS  - 0091-679X (Linking)
VI  - 104
DP  - 2011
TI  - Molecular cytogenetic methodologies and a BAC probe panel resource for genomic 
      analyses in the zebrafish.
PG  - 237-57
LID - 10.1016/B978-0-12-374814-0.00014-8 [doi]
AB  - Molecular cytogenetics is a field that emerged in the 1980s, based on a technique 
      referred to as fluorescence in situ hybridization, (FISH). Using FISH 
      methodologies, a specific DNA sequence or collection of DNA fragments may be 
      selectively labeled with a hapten molecule or fluorescent dye and hybridized to 
      denatured chromosomes, interphase cells, or even chromatin fibers. DNA 
      hybridization kinetics permit these labeled probes to anneal to their 
      complementary sequences on such chromosomal DNA preparations allowing for direct 
      visualization of the sequence of interest in the genome being interrogated. If 
      present, the relative chromosomal position of the sequence can sometimes also be 
      ascertained. Progress in molecular cytogenetic research has advanced the genetic 
      characterization of zebrafish models of human diseases as well as assisted with 
      accurate annotation of the zebrafish reference genome by anchoring large DNA 
      fragments to specific chromosome regions. Using the procedures described in this 
      chapter, hundreds of ambiguous zebrafish bacterial artificial chromosome (BAC) 
      clones have already been assigned to individual genetic linkage groups. Molecular 
      cytogenetic techniques can also be used to study gene duplication events and 
      study the molecular mechanisms by which they arise. Moreover, the availability of 
      a new molecular cytogenetic technique, array-based comparative genomic 
      hybridization (aCGH), is now able to identify gains and losses of DNA segments in 
      zebrafish DNA samples in a genome-wide manner and in a single assay.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Dobrinski, Kimberly P
AU  - Dobrinski KP
AD  - Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts, 
      USA; Harvard Medical School, Boston, Massachusetts, USA.
FAU - Brown, Kim H
AU  - Brown KH
FAU - Freeman, Jennifer L
AU  - Freeman JL
FAU - Lee, Charles
AU  - Lee C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Cell Biol
JT  - Methods in cell biology
JID - 0373334
RN  - 0 (DNA Probes)
SB  - IM
MH  - Animals
MH  - Chromosomes/*genetics
MH  - *Chromosomes, Artificial, Bacterial
MH  - Comparative Genomic Hybridization/*methods
MH  - DNA Probes/biosynthesis
MH  - Genome
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Larva/genetics
MH  - Metaphase
MH  - Zebrafish/*genetics
EDAT- 2011/09/20 06:00
MHDA- 2012/01/06 06:00
CRDT- 2011/09/20 06:00
PHST- 2011/09/20 06:00 [entrez]
PHST- 2011/09/20 06:00 [pubmed]
PHST- 2012/01/06 06:00 [medline]
AID - B978-0-12-374814-0.00014-8 [pii]
AID - 10.1016/B978-0-12-374814-0.00014-8 [doi]
PST - ppublish
SO  - Methods Cell Biol. 2011;104:237-57. doi: 10.1016/B978-0-12-374814-0.00014-8.