PMID- 21924304
OWN - NLM
STAT- MEDLINE
DCOM- 20120504
LR  - 20211020
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 163
IP  - 1
DP  - 2012 Jan
TI  - Cis-acting structural element in 5' UTR is essential for infectivity of porcine 
      reproductive and respiratory syndrome virus.
PG  - 108-19
LID - 10.1016/j.virusres.2011.08.018 [doi]
AB  - It is believed that the genomic 5' untranslated region (UTR) of Arterivirus plays 
      crucial roles in viral genomic replication, subgenomic mRNA transcription and 
      protein translation, yet the structure and function still remain largely unknown. 
      In this study, we conducted serial nucleotide truncation, ranging from 1 to 190 
      nucleotides, to the 5' UTR of the porcine reproductive and respiratory syndrome 
      virus (PRRSV) infectious full-length cDNA clone pAPRRS. In vitro synthetic RNAs 
      were transfected into MARC-145 cells for further genetic and virologic analysis. 
      Our results demonstrated that the first three nucleotides of PRRSV 5' UTR were 
      dispensable for virus viability, which however was repaired with foreign 
      sequences. In order to assess if the primary sequence or structural element play 
      more important regulatory roles, the CMV promoter-driven 5' UTR truncation mutant 
      cDNA clones were directly transfected into the BHK-21 cell lines. We found that 
      PRRSV tolerated the first 16 nucleotides sequence alteration of 5' UTR without 
      losing virus viability. However, these revertant viruses contained a range of 
      non-templated with unknown origin exogenous nucleotides in the repaired 5' end. 
      Further analyses revealed that the 5' proximal stem-loop 1 (SL1) in the highly 
      structured 5' UTR was invariably required for virus infectivity. Taken together, 
      we conclude that authentic 5'-proximal primary sequence is nonessential, but the 
      resultant structural elements are probably indispensable for PRRSV infectivity.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Gao, Fei
AU  - Gao F
AD  - College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 
      Province 210095, PR China.
FAU - Lu, Jiaqi
AU  - Lu J
FAU - Yao, Huochun
AU  - Yao H
FAU - Wei, Zuzhang
AU  - Wei Z
FAU - Yang, Qian
AU  - Yang Q
FAU - Yuan, Shishan
AU  - Yuan S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110908
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (5' Untranslated Regions)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - *5' Untranslated Regions
MH  - Animals
MH  - Cell Line
MH  - Cricetinae
MH  - DNA Mutational Analysis
MH  - Haplorhini
MH  - Microbial Viability
MH  - *Nucleic Acid Conformation
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*pathogenicity
MH  - RNA, Viral/*genetics
MH  - Sequence Deletion
MH  - Virulence
PMC - PMC7114472
EDAT- 2011/09/20 06:00
MHDA- 2012/05/05 06:00
PMCR- 2011/09/07
CRDT- 2011/09/20 06:00
PHST- 2011/03/16 00:00 [received]
PHST- 2011/08/30 00:00 [revised]
PHST- 2011/08/31 00:00 [accepted]
PHST- 2011/09/20 06:00 [entrez]
PHST- 2011/09/20 06:00 [pubmed]
PHST- 2012/05/05 06:00 [medline]
PHST- 2011/09/07 00:00 [pmc-release]
AID - S0168-1702(11)00324-8 [pii]
AID - 10.1016/j.virusres.2011.08.018 [doi]
PST - ppublish
SO  - Virus Res. 2012 Jan;163(1):108-19. doi: 10.1016/j.virusres.2011.08.018. Epub 2011 
      Sep 8.