PMID- 21925184
OWN - NLM
STAT- MEDLINE
DCOM- 20120209
LR  - 20111017
IS  - 1096-0805 (Electronic)
IS  - 0022-2011 (Linking)
VI  - 108
IP  - 3
DP  - 2011 Nov
TI  - Histopathological characterization and in situ detection of Callinectes sapidus 
      reovirus.
PG  - 226-8
LID - 10.1016/j.jip.2011.08.010 [doi]
AB  - A reovirus (tentatively designated as Callinectes sapidus reovirus, CsRV) was 
      found in the blue crabs C. sapidus collected in Chesapeake Bay in 2005. 
      Histological examination of hepatopancreas and gill from infected crabs revealed 
      eosinophilic to basophilic, cytoplasmic, inclusions in hemocytes and in cells of 
      connective tissue. A cDNA library was constructed from total RNA extracted from 
      hemolymph of infected crabs. One clone (designated as CsRV-28) with a 532-bp 
      insert was 75% identical in nucleotide sequence (and 95% similar in translated 
      amino acid sequence) to the quanylytransferase gene of the Scylla serrata 
      reovirus (SsRV). The insert of CsRV-28 was labeled with digoxigenin-11-dUTP and 
      hybridized to sections of hepatopancreas and gill of infected C. sapidus, this 
      probe reacted to hemocytes and cells in the connective tissue. No reaction was 
      seen in any of the tissues prepared from uninfected crabs. Thus, this in situ 
      hybridization procedure can be used to diagnose CsRV.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Tang, Kathy F J
AU  - Tang KF
AD  - Department of Veterinary Science and Microbiology, University of Arizona, Tucson, 
      AZ 85721, USA. fengjyu@u.arizona.edu
FAU - Messick, Gretchen A
AU  - Messick GA
FAU - Pantoja, Carlos R
AU  - Pantoja CR
FAU - Redman, Rita M
AU  - Redman RM
FAU - Lightner, Donald V
AU  - Lightner DV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20110908
PL  - United States
TA  - J Invertebr Pathol
JT  - Journal of invertebrate pathology
JID - 0014067
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Brachyura/*virology
MH  - Gills/pathology/virology
MH  - Hemocytes/pathology/virology
MH  - Hemolymph/cytology/virology
MH  - Hepatopancreas/pathology/virology
MH  - RNA, Viral/analysis
MH  - Reoviridae/*isolation & purification
MH  - Reoviridae Infections/pathology/*veterinary
EDAT- 2011/09/20 06:00
MHDA- 2012/02/10 06:00
CRDT- 2011/09/20 06:00
PHST- 2011/06/29 00:00 [received]
PHST- 2011/08/30 00:00 [revised]
PHST- 2011/08/31 00:00 [accepted]
PHST- 2011/09/20 06:00 [entrez]
PHST- 2011/09/20 06:00 [pubmed]
PHST- 2012/02/10 06:00 [medline]
AID - S0022-2011(11)00197-2 [pii]
AID - 10.1016/j.jip.2011.08.010 [doi]
PST - ppublish
SO  - J Invertebr Pathol. 2011 Nov;108(3):226-8. doi: 10.1016/j.jip.2011.08.010. Epub 
      2011 Sep 8.