PMID- 21925978
OWN - NLM
STAT- MEDLINE
DCOM- 20111207
LR  - 20191210
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 879
IP  - 28
DP  - 2011 Oct 15
TI  - Extraction and determination of opium alkaloids in urine samples using dispersive 
      liquid-liquid microextraction followed by high-performance liquid chromatography.
PG  - 2978-83
LID - 10.1016/j.jchromb.2011.08.033 [doi]
AB  - A simple, rapid and sensitive method based on dispersive liquid-liquid 
      microextraction (DLLME) combined with high-performance liquid 
      chromatography-ultraviolet detection (HPLC-UV) was used to determine opium 
      alkaloids in urine samples. Some effective parameters on extraction were studied 
      and optimized. Under the optimum conditions, enrichment factors and recoveries 
      for different opiates are in the range of 63.0-104.5 and 31.5-52.2%, 
      respectively. The calibration graphs are linear in the range of 0.50-500 mug L(-1) 
      and limit of detections (LODs) are in the range of 0.2-10 mug L(-1). The relative 
      standard deviations (RSDs) for 200 mug L(-1) of morphine, codeine and thebaine, 
      5.0 mug L(-1) of papaverine and 10.0 mug L(-1) of noscapine in diluted urine sample 
      are in the range of 2.8-6.1% (n=7). The relative recoveries of urine samples 
      spiked with alkaloids are 84.3-106.0%. The obtained results show that DLLME 
      combined with HPLC-UV is a fast and simple method for the determination of opium 
      alkaloids in urine samples.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Shamsipur, Mojtaba
AU  - Shamsipur M
AD  - Department Chemistry, Razi University, Kermanshah, Iran. mshamsipur@yahoo.com
FAU - Fattahi, Nazir
AU  - Fattahi N
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110831
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Opiate Alkaloids)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Liquid Phase Microextraction/*methods
MH  - Opiate Alkaloids/isolation & purification/*urine
MH  - Sensitivity and Specificity
EDAT- 2011/09/20 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/09/20 06:00
PHST- 2011/06/13 00:00 [received]
PHST- 2011/08/24 00:00 [revised]
PHST- 2011/08/26 00:00 [accepted]
PHST- 2011/09/20 06:00 [entrez]
PHST- 2011/09/20 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - S1570-0232(11)00559-9 [pii]
AID - 10.1016/j.jchromb.2011.08.033 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Oct 15;879(28):2978-83. doi: 
      10.1016/j.jchromb.2011.08.033. Epub 2011 Aug 31.