PMID- 21927668
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20111110
LR  - 20211020
IS  - 2005-1212 (Electronic)
IS  - 1976-2283 (Print)
IS  - 1976-2283 (Linking)
VI  - 5
IP  - 3
DP  - 2011 Sep
TI  - Proliferation of Hepatic Oval Cells via Cyclooxygenase-2 and Extracellular Matrix 
      Protein Signaling during Liver Regeneration Following 2-AAF/Partial Hepatectomy 
      in Rats.
PG  - 367-76
LID - 10.5009/gnl.2011.5.3.367 [doi]
AB  - BACKGROUND/AIMS: In the 2-acetylaminofluorene (2-AAF)/70% partial hepatectomy 
      (PHx) model, the mechanism underlying the differentiation of activated hepatic 
      oval cells (HOCs) into hepatocytes and bile ductile cells is unclear. We 
      investigated the role of cyclooxygenase-2 (COX-2) in HOCs and the relationship 
      between COX-2 and extracellular matrix proteins in cellular proliferation. 
      METHODS: Reverse transcription-polymerase chain reaction, immunohistochemical 
      staining, and Western blotting were used to assess COX-2 expression. The 
      co-localization of COX-2 with Thy1, c-Met, epithelial cell adhesion molecule, and 
      alpha-smooth muscle actin was also examined. Additionally, we investigated whether 
      connective tissue growth factor (CTGF), fibronectin (FN), extracellular 
      signal-regulated kinase 1/2 (P-ERK1/2), and AKT were expressed in HOCs. RESULTS: 
      The expression of COX-2, prostaglandin E2 receptors, and c-Met was upregulated in 
      HOCs. However, HOCs treated with the COX-2 inhibitor NS398 showed decreased 
      COX-2, CTGF, FN, and AKT expression, whereas P-ERK1/2 was unaffected. 
      Additionally, NS398 inhibited HOC proliferation, but not the proliferation of 
      HOCs cultured on FN-coated dishes. Furthermore, the proliferative response of 
      HOCs treated with NS398 was reversed by hepatic growth factor treatment. 
      CONCLUSIONS: These results suggest that HOC proliferation is mediated through 
      COX-2, extracellular FN expression, and AKT activation. Thus, COX-2 plays an 
      important role in HOC proliferation following acute injury.
FAU - Bae, Si Hyun
AU  - Bae SH
AD  - Department of Internal Medicine, The Catholic University of Korea, College of 
      Medicine, Seoul, Korea.
FAU - Oh, Seh Hoon
AU  - Oh SH
FAU - Yoon, Seung Kew
AU  - Yoon SK
FAU - Park, Joung Ah
AU  - Park JA
FAU - Kim, Gi Dae
AU  - Kim GD
FAU - Hur, Wonhee
AU  - Hur W
FAU - Choi, Jong Young
AU  - Choi JY
FAU - Oh, Il Hoan
AU  - Oh IH
FAU - Yoon, Kun Ho
AU  - Yoon KH
LA  - eng
PT  - Journal Article
DEP - 20110818
PL  - Korea (South)
TA  - Gut Liver
JT  - Gut and liver
JID - 101316452
PMC - PMC3166680
OTO - NOTNLM
OT  - Cyclooxygenase-2
OT  - Hepatic oval cells
OT  - Liver generation
COIS- No potential conflict of interest relevant to this article was reported.
EDAT- 2011/09/20 06:00
MHDA- 2011/09/20 06:01
PMCR- 2011/09/01
CRDT- 2011/09/20 06:00
PHST- 2010/12/10 00:00 [received]
PHST- 2011/04/27 00:00 [accepted]
PHST- 2011/09/20 06:00 [entrez]
PHST- 2011/09/20 06:00 [pubmed]
PHST- 2011/09/20 06:01 [medline]
PHST- 2011/09/01 00:00 [pmc-release]
AID - 10.5009/gnl.2011.5.3.367 [doi]
PST - ppublish
SO  - Gut Liver. 2011 Sep;5(3):367-76. doi: 10.5009/gnl.2011.5.3.367. Epub 2011 Aug 18.