PMID- 21927816
OWN - NLM
STAT- MEDLINE
DCOM- 20120504
LR  - 20220309
IS  - 1559-0283 (Electronic)
IS  - 1085-9195 (Linking)
VI  - 62
IP  - 1
DP  - 2012 Jan
TI  - Intratumoral heterogeneity determines discordant results of diagnostic tests for 
      human epidermal growth factor receptor (HER) 2 in gastric cancer specimens.
PG  - 221-8
LID - 10.1007/s12013-011-9286-1 [doi]
AB  - Accurate assessment of human epidermal growth factor receptor (HER) 2 is 
      essential for efficient selection of patients who may benefit from therapies 
      targeting this surface receptor (e.g., trastuzumab). Intratumoral heterogeneity 
      of HER2 expression may potentially contribute to inaccurate assessment of HER2 
      status. To clarify intratumoral heterogeneity of HER2 expression and its 
      potential clinical impact on assessment of HER2 status, we analyzed 148 
      endoscopic biopsy specimens and 117 excisional tumor specimens collected from 148 
      patients with primary gastric cancer. Specifically, we assessed HER2 protein 
      overexpression and gene amplification using, respectively, immunohistochemistry 
      (IHC) and fluorescence in situ hybridization (FISH). There were 28 IHC-positive 
      cases and 25 FISH-positive cases among these 148 patients. Heterogeneous HER2 
      protein expression was demonstrated in 23 of 29 (79.3%) IHC-positive cases, while 
      gene expression heterogeneity was found in 11 of 25 (44.0%) FISH-positive cases. 
      Intratumoral heterogeneity was the main reason of discordant results between IHC 
      and FISH or between endoscopic biopsy and excisional tumor specimens. The 
      clinical significance and impact of intratumoral HER2 expression heterogeneity on 
      treatment outcome in gastric cancer require further studies.
FAU - Yang, Jiyuan
AU  - Yang J
AD  - Department of Digestion, Renmin Hospital of WuHan University, HuBei, WuHan, 
      China.
FAU - Luo, Hesheng
AU  - Luo H
FAU - Li, Yan
AU  - Li Y
FAU - Li, Junchuan
AU  - Li J
FAU - Cai, Zhiqiang
AU  - Cai Z
FAU - Su, Xiaoyan
AU  - Su X
FAU - Dai, Danqing
AU  - Dai D
FAU - Du, Wei
AU  - Du W
FAU - Chen, Tingxuan
AU  - Chen T
FAU - Chen, Ming
AU  - Chen M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cell Biochem Biophys
JT  - Cell biochemistry and biophysics
JID - 9701934
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Receptor, ErbB-2/genetics/*metabolism
MH  - Reproducibility of Results
MH  - Stomach Neoplasms/*diagnosis/pathology
EDAT- 2011/09/20 06:00
MHDA- 2012/05/05 06:00
CRDT- 2011/09/20 06:00
PHST- 2011/09/20 06:00 [entrez]
PHST- 2011/09/20 06:00 [pubmed]
PHST- 2012/05/05 06:00 [medline]
AID - 10.1007/s12013-011-9286-1 [doi]
PST - ppublish
SO  - Cell Biochem Biophys. 2012 Jan;62(1):221-8. doi: 10.1007/s12013-011-9286-1.