PMID- 21928345
OWN - NLM
STAT- MEDLINE
DCOM- 20120419
LR  - 20131121
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 227
IP  - 6
DP  - 2012 Jun
TI  - Activity of matrix metallo proteinases (MMPs) and the tissue inhibitor of MMP 
      (TIMP)-1 in electromagnetic field-exposed THP-1 cells.
PG  - 2767-74
LID - 10.1002/jcp.23024 [doi]
AB  - Matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) are the 
      main determinants of tissue remodeling in both physiological and pathological 
      processes. Metabolic processes, which generate oxidants and antioxidants can be 
      influenced by environmental factors such as electromagnetic fields (EMF). We 
      analyzed the effects of EMF on the activity and expression of MMPs in THP-1 
      cells. Cells were exposed to a 50 Hz, 1 mT EMF for 24 h and incubated with or 
      without LPS. Our data indicate that THP-1 cells exposed to EMF causes a reduction 
      of anti-oxidant enzyme activity and an enhancement of nitrogen intermediates 
      involving the iNOS pathway. We then analyzed the role of nitration of TIMP-1 in 
      increasing the activity of MMPs in EMF exposed cells. Molecular modeling tools 
      were employed to identify the most plausible sites in the active conformation of 
      TIMP-1; at least two protein sites, Y120 and Y38 and/or Y72 were identified. 
      Reactive nitrogen species (RNS) may affect protein targets, such as TIMP-1, which 
      are crucial for the regulation of MMP activities by oxidation of sulfydryl 
      groups, or by nitration of tyrosine residues. These results may suggest a pathway 
      connecting an imbalance of MMPs and their cognate inhibitor TIMP-1.
CI  - Copyright (c) 2011 Wiley Periodicals, Inc.
FAU - Patruno, Antonia
AU  - Patruno A
AD  - Department of Drug Sciences, University G. d'Annunzio, Chieti, Italy. 
      antoniapatruno@unich.it
FAU - Pesce, Mirko
AU  - Pesce M
FAU - Marrone, Alessandro
AU  - Marrone A
FAU - Speranza, Lorenza
AU  - Speranza L
FAU - Grilli, Alfredo
AU  - Grilli A
FAU - De Lutiis, Maria Anna
AU  - De Lutiis MA
FAU - Felaco, Mario
AU  - Felaco M
FAU - Reale, Marcella
AU  - Reale M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Antioxidants)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Nitrogen Species)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (TIMP1 protein, human)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 42HK56048U (Tyrosine)
RN  - EC 1.14.13.39 (NOS2 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 3.4.24.24 (MMP2 protein, human)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Antioxidants/metabolism
MH  - Cell Line, Tumor
MH  - *Electromagnetic Fields
MH  - Gene Expression Regulation, Enzymologic
MH  - Humans
MH  - Leukemia, Myeloid, Acute/*enzymology
MH  - Lipopolysaccharides/pharmacology
MH  - Matrix Metalloproteinase 2/genetics/*metabolism
MH  - Matrix Metalloproteinase 9/genetics/*metabolism
MH  - Models, Molecular
MH  - Nitric Oxide Synthase Type II/metabolism
MH  - Protein Processing, Post-Translational
MH  - RNA, Messenger/metabolism
MH  - Reactive Nitrogen Species/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Time Factors
MH  - Tissue Inhibitor of Metalloproteinase-1/chemistry/genetics/*metabolism
MH  - Tyrosine
EDAT- 2011/09/20 06:00
MHDA- 2012/04/20 06:00
CRDT- 2011/09/20 06:00
PHST- 2011/09/20 06:00 [entrez]
PHST- 2011/09/20 06:00 [pubmed]
PHST- 2012/04/20 06:00 [medline]
AID - 10.1002/jcp.23024 [doi]
PST - ppublish
SO  - J Cell Physiol. 2012 Jun;227(6):2767-74. doi: 10.1002/jcp.23024.