PMID- 21928379
OWN - NLM
STAT- MEDLINE
DCOM- 20120327
LR  - 20191210
IS  - 1554-527X (Electronic)
IS  - 0736-0266 (Linking)
VI  - 30
IP  - 4
DP  - 2012 Apr
TI  - Age-related expression of MCP-1 and MMP-3 in mouse intervertebral disc in 
      relation to TWEAK and TNF-alpha stimulation.
PG  - 599-605
LID - 10.1002/jor.21560 [doi]
AB  - This study was undertaken to investigate the age-related differences of monocyte 
      chemotactic protein-1 (MCP-1) and matrix metalloproteinase-3 (MMP-3) expression 
      in mouse intervertebral disc (IVD) and to determine whether MMP-3 plays a role in 
      disc degeneration. Expression of MCP-1 and MMP-3 mRNA in mouse IVD was assessed 
      by quantitative PCR. The ability of MCP-1 and MMP-3 expression in IVD to respond 
      to TNF-alpha or TWEAK stimulation was examined by quantitative PCR, WB, ELISA, and 
      immunohistochemistry. IVD derived from MMP-3-deficient and wild-type mice were 
      compared using Safranin-O staining and immunohistochemistry. mRNA levels of MCP-1 
      and MMP-3 in IVD significantly diminished and the ability of MCP-1 or MMP-3 
      expression to respond to TNF-alpha or TWEAK stimulation was significantly reduced as 
      age increased. IVD derived from 64-week-old wild-type mice showed clearly diffuse 
      proteoglycan loss by Safranin-O staining and immunohistochemistry compared with 
      younger mice. However, no loss of proteoglycan and typeII collagen were observed 
      in IVD derived from 64-week-old MMP-3-deficient mice. MCP-1 and MMP-3 expression 
      in mouse IVD showed age-related decreases. The response to inflammation in IVD 
      also displayed age-related changes. Therefore, disc degeneration may vary with 
      the patients' age and targeting MMP-3 may be a possible future therapeutic 
      strategy for disc degeneration.
CI  - Copyright (c) 2011 Orthopaedic Research Society.
FAU - Fujita, Koji
AU  - Fujita K
AD  - Department of Orthopaedic Surgery, University of Yamanashi, 1110 Shimokato, Chuo, 
      Yamanashi 409-3898, Japan.
FAU - Ando, Takashi
AU  - Ando T
FAU - Ohba, Tetsuro
AU  - Ohba T
FAU - Wako, Masanori
AU  - Wako M
FAU - Sato, Nobutaka
AU  - Sato N
FAU - Nakamura, Yuki
AU  - Nakamura Y
FAU - Ohnuma, Yuko
AU  - Ohnuma Y
FAU - Hara, Yasushi
AU  - Hara Y
FAU - Kato, Ryohei
AU  - Kato R
FAU - Nakao, Atsuhito
AU  - Nakao A
FAU - Haro, Hirotaka
AU  - Haro H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110916
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (Acan protein, mouse)
RN  - 0 (Aggrecans)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokine TWEAK)
RN  - 0 (Proteoglycans)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tnfsf12 protein, mouse)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Tumor Necrosis Factors)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (vascular endothelial growth factor A, mouse)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.17 (Mmp3 protein, mouse)
SB  - IM
MH  - Aggrecans/genetics/immunology/metabolism
MH  - Aging/pathology/*physiology
MH  - Animals
MH  - Chemokine CCL2/*genetics/immunology/metabolism
MH  - Cytokine TWEAK
MH  - Gene Expression/drug effects/immunology
MH  - Intervertebral Disc/immunology/metabolism/pathology
MH  - Intervertebral Disc Degeneration/immunology/metabolism/*physiopathology
MH  - Matrix Metalloproteinase 3/*genetics/immunology/metabolism
MH  - Mice
MH  - Mice, 129 Strain
MH  - Mice, Inbred C57BL
MH  - Mice, Mutant Strains
MH  - Proteoglycans/biosynthesis
MH  - Recombinant Proteins/pharmacology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Tumor Necrosis Factors/*pharmacology
MH  - Vascular Endothelial Growth Factor A/genetics
EDAT- 2011/09/20 06:00
MHDA- 2012/03/28 06:00
CRDT- 2011/09/20 06:00
PHST- 2011/03/12 00:00 [received]
PHST- 2011/08/30 00:00 [accepted]
PHST- 2011/09/20 06:00 [entrez]
PHST- 2011/09/20 06:00 [pubmed]
PHST- 2012/03/28 06:00 [medline]
AID - 10.1002/jor.21560 [doi]
PST - ppublish
SO  - J Orthop Res. 2012 Apr;30(4):599-605. doi: 10.1002/jor.21560. Epub 2011 Sep 16.