PMID- 21931959
OWN - NLM
STAT- MEDLINE
DCOM- 20120322
LR  - 20211020
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 360
IP  - 1-2
DP  - 2012 Jan
TI  - Molecular and functional characterization of glycogen synthase in the porcine 
      satellite cells under insulin treatment.
PG  - 169-80
LID - 10.1007/s11010-011-1054-4 [doi]
AB  - Glycogen synthase (GS) catalyzes the key step of glycogen synthesis and plays an 
      important role in glycogen metabolism in liver and muscle. In this study, we 
      cloned the cDNA and promoter sequences of porcine glycogen synthesis genes (GYS1 
      and GYS2). Expression analysis revealed that porcine GYS1 was highly expressed in 
      the skeletal muscle and heart. GYS2 was expressed specifically in liver and 
      subcutaneous adipose tissue. The expression level of GYS1 was up-regulated from 
      proliferation to differentiation in the porcine satellite cells, and insulin did 
      not significantly affect the transcription of GYS1. Insulin stimulated 
      72-h-differentiated satellite cells as indicated by decrease in phosphorylation 
      of GS, but did not affect GYS1 transcription and total GS protein level, 
      suggesting that the effect of insulin is primarily mediated via 
      posttranscriptional control rather than regulated at the transcriptional level. 
      Four single-nucleotide polymorphisms (SNPs) were detected in the promoter and 
      cDNA sequences of porcine GYS1. Association analyses revealed that the GYS1 Hin6I 
      and MvaI polymorphisms both had significant associations (P < 0.05) with pH of M. 
      longissimus dorsi (pHLD), M. biceps femoris (pHBF) and M. semipinalis capitis 
      (pHSC) at 45 min postmortem. These results provide useful information for further 
      investigation on the function of glycogen synthase in porcine skeletal muscle.
FAU - Wang, Linjie
AU  - Wang L
AD  - Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College 
      of Animal Science, Huazhong Agricultural University, Wuhan, Hubei, People's 
      Republic of China.
FAU - Xiong, Yuanzhu
AU  - Xiong Y
FAU - Zuo, Bo
AU  - Zuo B
FAU - Lei, Minggang
AU  - Lei M
FAU - Ren, Zhuqing
AU  - Ren Z
FAU - Xu, Dequan
AU  - Xu D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110920
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Insulin)
RN  - EC 2.4.1.11 (Glycogen Synthase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Enzyme Activation
MH  - Female
MH  - Gene Expression
MH  - Gene Expression Regulation
MH  - Gene Frequency
MH  - Genetic Association Studies
MH  - Glycogen Synthase/genetics/*metabolism
MH  - Insulin/*pharmacology/physiology
MH  - Liver/enzymology
MH  - Male
MH  - Meat/standards
MH  - Molecular Sequence Data
MH  - Muscle, Skeletal/cytology/enzymology
MH  - Myocardium/enzymology
MH  - Organ Specificity
MH  - Phosphorylation
MH  - Polymorphism, Single Nucleotide
MH  - Promoter Regions, Genetic
MH  - Satellite Cells, Skeletal Muscle/drug effects/*enzymology
MH  - Sequence Analysis, DNA
MH  - Subcutaneous Fat/enzymology
MH  - *Sus scrofa
EDAT- 2011/09/21 06:00
MHDA- 2012/03/23 06:00
CRDT- 2011/09/21 06:00
PHST- 2011/06/13 00:00 [received]
PHST- 2011/09/08 00:00 [accepted]
PHST- 2011/09/21 06:00 [entrez]
PHST- 2011/09/21 06:00 [pubmed]
PHST- 2012/03/23 06:00 [medline]
AID - 10.1007/s11010-011-1054-4 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2012 Jan;360(1-2):169-80. doi: 10.1007/s11010-011-1054-4. Epub 
      2011 Sep 20.