PMID- 21932134
OWN - NLM
STAT- MEDLINE
DCOM- 20120511
LR  - 20240426
IS  - 1432-0851 (Electronic)
IS  - 0340-7004 (Print)
IS  - 0340-7004 (Linking)
VI  - 61
IP  - 3
DP  - 2012 Mar
TI  - Inclusive estimation of complex antigen presentation functions of 
      monocyte-derived dendritic cells differentiated under normoxia and hypoxia 
      conditions.
PG  - 409-24
LID - 10.1007/s00262-011-1112-5 [doi]
AB  - Dendritic cells (DCs) generated from monocytes under 20% O2 are now used as 
      therapeutic tools for cancer patients. However, the O2 concentration is between 3 
      and 0.5% in most tissues. We evaluated these complicated functions of DCs under 
      oxygen tensions mimicking in vivo situations. Immature DCs (imDCs) were generated 
      from monocytes using IL-4 and GM-CSF under normoxia (20% O2; N-imDCs) or hypoxia 
      (1% O2; H-imDCs). Mature DCs (mDCs) were induced with LPS. DCs were further 
      exposed to normoxia (N/N-DCs) or hypoxia (N/H-DCs and H/H-DCs) conditions. Using 
      a 2-D culture system, H-DCs were smaller in size than N-DCs, and H/H-DCs 
      exhibited higher allo-T cell stimulation ability than N/N-DCs and N/H-DCs. On the 
      other hand, motility and phagocytic ability of H/H-DCs were significantly lower 
      than those of N/H-DCs and N/N-DCs. In a 3-D culture system, however, maturation 
      of H/H-imDCs and N/H-imDCs was suppressed compared with N/N-imDCs as a result of 
      their decreased motility and phagocytosis. Interestingly, silencing of HIF-1alpha by 
      RNA interference decreased CD83 expression without affecting any antigen 
      presentation abilities except for the ability to stimulate the allo-T cell 
      population. Our data could help our understanding of DCs, especially therapeutic 
      DCs, in vivo.
FAU - Ogino, Toshitatsu
AU  - Ogino T
AD  - Department of Cancer Therapy and Research, Graduate School of Medical Sciences, 
      Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
FAU - Onishi, Hideya
AU  - Onishi H
FAU - Suzuki, Hiroyuki
AU  - Suzuki H
FAU - Morisaki, Takashi
AU  - Morisaki T
FAU - Tanaka, Masao
AU  - Tanaka M
FAU - Katano, Mitsuo
AU  - Katano M
LA  - eng
PT  - Journal Article
DEP - 20110920
PL  - Germany
TA  - Cancer Immunol Immunother
JT  - Cancer immunology, immunotherapy : CII
JID - 8605732
RN  - 0 (Antigens, CD)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Immunoglobulins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Antigen Presentation/*immunology
MH  - Antigens, CD/immunology/metabolism
MH  - Cell Culture Techniques
MH  - Cell Differentiation/drug effects/*immunology
MH  - Cell Movement/immunology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/metabolism
MH  - Flow Cytometry
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - Hypoxia
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/genetics/immunology/metabolism
MH  - Immunoblotting
MH  - Immunoglobulins/immunology/metabolism
MH  - Interleukin-4/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Membrane Glycoproteins/immunology/metabolism
MH  - Models, Immunological
MH  - Monocytes/*immunology/metabolism
MH  - Oxygen/pharmacology
MH  - Phagocytosis/immunology
MH  - RNA Interference
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - T-Lymphocytes/immunology/metabolism
MH  - CD83 Antigen
PMC - PMC11029581
EDAT- 2011/09/21 06:00
MHDA- 2012/05/12 06:00
PMCR- 2011/09/20
CRDT- 2011/09/21 06:00
PHST- 2011/05/10 00:00 [received]
PHST- 2011/09/06 00:00 [accepted]
PHST- 2011/09/21 06:00 [entrez]
PHST- 2011/09/21 06:00 [pubmed]
PHST- 2012/05/12 06:00 [medline]
PHST- 2011/09/20 00:00 [pmc-release]
AID - 1112 [pii]
AID - 10.1007/s00262-011-1112-5 [doi]
PST - ppublish
SO  - Cancer Immunol Immunother. 2012 Mar;61(3):409-24. doi: 10.1007/s00262-011-1112-5. 
      Epub 2011 Sep 20.