PMID- 21933042
OWN - NLM
STAT- MEDLINE
DCOM- 20120803
LR  - 20191210
IS  - 1029-2403 (Electronic)
IS  - 1026-8022 (Linking)
VI  - 53
IP  - 3
DP  - 2012 Mar
TI  - Detection of BCR-ABL fusion proteins in patients with leukemia using a cytometric 
      bead array.
PG  - 451-5
LID - 10.3109/10428194.2011.625100 [doi]
AB  - Rapid and accurate detection of BCR-ABL fusion proteins is vital for the 
      diagnosis and classification of leukemias, especially chronic myeloid leukemia 
      (CML) and acute lymphoblastic leukemia (ALL). Recently, the cytometric bead array 
      (CBA) system has been used to identify BCR-ABL proteins in leukemic cell lysates. 
      To evaluate the accuracy of this new technique, we tested 70 patients with 
      hematological diseases using the CBA system, cytogenetic analysis, fluorescence 
      in situ hybridization (FISH) and real-time quantitative polymerase chain reaction 
      (RQ-PCR). Most of the results obtained by the CBA system were concordant with 
      those by cytogenetic analysis, FISH and RQ-PCR. In the CBA system, dilution 
      experiments with positive control samples revealed sensitivities of at least 1%. 
      The statistical analysis revealed that the mean fluorescence intensity (MFI) 
      values of the BCR-ABL fusion proteins from mononuclear cells (MNCs) were higher 
      than those from WBC or BM samples. The MFI values of cells were not dramatically 
      reduced after 24 hours of storage at room temperature. The CBA technique detected 
      all types of BCR-ABL proteins in leukemic cells with high specificity and 
      sensitivity. Therefore, CBAs will contribute to the fast and easy diagnosis of 
      leukemias, the classification of leukemias and the monitoring of minimal residual 
      disease (MRD).
FAU - Yujie, Wu
AU  - Yujie W
AD  - Department of Hematology, The First Affiliated Hospital of Nanjing Medical 
      University, Jiangsu Province Hospital, Nanjing, Jiangsu, China. 
      wuyujie123456@163.com
FAU - Yu, Zhu
AU  - Yu Z
FAU - Sixuan, Qian
AU  - Sixuan Q
FAU - Li, Wang
AU  - Li W
FAU - Peng, Liu
AU  - Peng L
FAU - Zeng, Ge
AU  - Zeng G
FAU - Sujiang, Zhang
AU  - Sujiang Z
FAU - Jianyong, Li
AU  - Jianyong L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20111128
PL  - United States
TA  - Leuk Lymphoma
JT  - Leukemia & lymphoma
JID - 9007422
RN  - 0 (Antibodies, Immobilized)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (RNA, Neoplasm)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - *Antibodies, Immobilized
MH  - Biomarkers, Tumor/*analysis/blood/immunology
MH  - Bone Marrow Cells/chemistry
MH  - Early Diagnosis
MH  - Female
MH  - Fluorescent Antibody Technique, Direct/*methods
MH  - Fusion Proteins, bcr-abl/*analysis/blood/immunology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL 
      Positive/blood/diagnosis/genetics/*metabolism
MH  - Leukocytes/chemistry
MH  - Male
MH  - Microspheres
MH  - Middle Aged
MH  - Monocytes/chemistry
MH  - Polycythemia Vera/blood/genetics/metabolism
MH  - Precursor Cell Lymphoblastic 
      Leukemia-Lymphoma/blood/diagnosis/genetics/*metabolism
MH  - RNA, Neoplasm/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Specimen Handling
MH  - Thrombocythemia, Essential/blood/genetics/metabolism
MH  - Young Adult
EDAT- 2011/09/22 06:00
MHDA- 2012/08/04 06:00
CRDT- 2011/09/22 06:00
PHST- 2011/09/22 06:00 [entrez]
PHST- 2011/09/22 06:00 [pubmed]
PHST- 2012/08/04 06:00 [medline]
AID - 10.3109/10428194.2011.625100 [doi]
PST - ppublish
SO  - Leuk Lymphoma. 2012 Mar;53(3):451-5. doi: 10.3109/10428194.2011.625100. Epub 2011 
      Nov 28.