PMID- 21933721
OWN - NLM
STAT- MEDLINE
DCOM- 20120213
LR  - 20131121
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 726
IP  - 2
DP  - 2011 Dec 24
TI  - In vivo radioprotective potential of thymol, a monoterpene phenol derivative of 
      cymene.
PG  - 136-45
LID - 10.1016/j.mrgentox.2011.08.007 [doi]
AB  - The radioprotective and anticlastogenic potential of a phenol derivative 
      monoterpene thymol(TOH), against whole-body gamma radiation was studied in Swiss 
      albino mice. Acute toxicity of TOH, with an LD(50(14)) of 1134.03mg/kgbwt., was 
      observed when administered intra-peritoneally (i.p.). The radioprotective 
      potential of TOH was evaluated using the optimal dose of 10mg/kgbwt. TOH, which 
      increased the LD(50/30) by 2.17Gy and resulted in a dose reduction factor (DRF) 
      of 1.25. A significant (p<0.01) reduction in micronucleated, polychromatic 
      erythrocytes (PCE), normochromatic erythrocytes (NCE), and an increased PCE/NCE 
      ratio was also observed after administration of 10mg/kg.b.wt. TOH prior to gamma 
      radiation, indicating its antigenotoxic effect. TOH pre-treatment significantly 
      (p<0.01) elevated reduced glutathione, glutathione-S-transferase, catalase, and 
      superoxide dismutase levels and decreased lipid peroxidation levels in mouse 
      liver homogenates at 24 and 48h after exposure to 4.5Gy of radiation. Further, 
      TOH treatment before exposure to 7.5Gy of gamma radiation resulted in a 
      significant (p<0.01) increase in hematological parameters at various 
      post-treatment time points, with increased numbers of endogenous spleen colonies 
      as well. The histological observations indicated a decline in villus heights and 
      crypt numbers in mouse jejunum and were accompanied by a significant decrease in 
      bone marrow nucleated cells in the irradiated group, which was almost normalized 
      by pre-treatment with TOH. Our study clearly documents the antioxidant, 
      anticlastogenic and radioprotective potentials of TOH, which may be attributed to 
      several possible mechanisms, such as normalization of intracellular antioxidant 
      levels and free radical scavenging activities by TOH.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - P R, Archana
AU  - P R A
AD  - Division of Radiobiology & Toxicology, Manipal University, Manipal, India.
FAU - Nageshwar Rao, B
AU  - Nageshwar Rao B
FAU - Satish Rao, B S
AU  - Satish Rao BS
LA  - eng
PT  - Journal Article
DEP - 20110914
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Antimutagenic Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Radiation-Protective Agents)
RN  - 3J50XA376E (Thymol)
SB  - IM
MH  - Animals
MH  - Antimutagenic Agents/*pharmacology
MH  - Antioxidants/*pharmacology
MH  - Blood Cells/drug effects
MH  - Bone Marrow Cells/drug effects
MH  - Gamma Rays
MH  - Intestinal Mucosa/drug effects
MH  - Lipid Peroxidation/drug effects
MH  - Mice
MH  - Micronuclei, Chromosome-Defective
MH  - Radiation-Protective Agents/*pharmacology
MH  - Thymol/*pharmacology/toxicity
EDAT- 2011/09/22 06:00
MHDA- 2012/02/14 06:00
CRDT- 2011/09/22 06:00
PHST- 2011/01/25 00:00 [received]
PHST- 2011/08/03 00:00 [revised]
PHST- 2011/08/26 00:00 [accepted]
PHST- 2011/09/22 06:00 [entrez]
PHST- 2011/09/22 06:00 [pubmed]
PHST- 2012/02/14 06:00 [medline]
AID - S1383-5718(11)00263-4 [pii]
AID - 10.1016/j.mrgentox.2011.08.007 [doi]
PST - ppublish
SO  - Mutat Res. 2011 Dec 24;726(2):136-45. doi: 10.1016/j.mrgentox.2011.08.007. Epub 
      2011 Sep 14.