PMID- 21933790
OWN - NLM
STAT- MEDLINE
DCOM- 20120112
LR  - 20130520
IS  - 1462-0332 (Electronic)
IS  - 1462-0324 (Linking)
VI  - 50
IP  - 12
DP  - 2011 Dec
TI  - Antigen-specific immature dendritic cell vaccine ameliorates anti-dsDNA 
      antibody-induced renal damage in a mouse model.
PG  - 2187-96
LID - 10.1093/rheumatology/ker231 [doi]
AB  - OBJECTIVES: Dendritic cells (DCs) can inhibit immune response by clonal anergy 
      when immature. Recent studies have shown that immature DCs (iDCs) may serve as a 
      live cell vaccine after specific antigen pulse based on its potential of blocking 
      antibody production. In this study, we aimed to investigate the effects of 
      nuclear antigen-pulsed iDCs in the treatment of lupus-like renal damages induced 
      by anti-dsDNA antibodies. METHODS: iDCs were generated from haemopoietic stem 
      cells in bone marrow and then pulsed in vitro with nuclear antigen. The iDC 
      vaccine and corresponding controls were injected into mice with lupus-like renal 
      damages. The evaluation of disease was monitored by biochemical parameters and 
      histological scores. Anti-dsDNA antibody isotypes and T-lymphocyte-produced 
      cytokines were analysed for elucidating therapeutic mechanisms. RESULTS; The mice 
      treated with antigen-pulsed iDCs had a sustained remission of renal damage 
      compared with those injected with non-pulsed iDCs or other controls, including 
      decreased anti-dsDNA antibody level, less proteinuria, lower blood urea nitrogen 
      and serum creatinine values, and improved histological evaluation. Analysis on 
      isotypes of anti-dsDNA antibody showed that iDC vaccine preferentially inhibited 
      the production of IgG3, IgG2b and IgG2a. Furthermore, administration of 
      antigen-treated iDCs to mice resulted in significantly reduced IL-2, IL-4 and 
      IL-12 and IFN-gamma produced by T-memory cells. Conversely, the vaccination of 
      antigen-pulsed mature DCs led to increased anti-dsDNA antibody production and an 
      aggravation of lupus-like disease in the model. CONCLUSIONS; These results 
      suggested the high potency of iDC vaccine in preventing lupus-like renal injuries 
      induced by pathogenic autoantibodies.
FAU - Xia, Yumin
AU  - Xia Y
AD  - Department of Dermatology, Renmin Hospital of Wuhan University, Wuhan, China. 
      ymxia@whu.edu.cn
FAU - Jiang, Shan
AU  - Jiang S
FAU - Weng, Shenhong
AU  - Weng S
FAU - Lv, Xiaochun
AU  - Lv X
FAU - Cheng, Hong
AU  - Cheng H
FAU - Fang, Chunhong
AU  - Fang C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110920
PL  - England
TA  - Rheumatology (Oxford)
JT  - Rheumatology (Oxford, England)
JID - 100883501
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Cytokines)
RN  - 0 (DNA, Protozoan)
RN  - 0 (Vaccines, DNA)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Antibodies, Antinuclear/*immunology
MH  - Cytokines/biosynthesis
MH  - DNA, Protozoan/*administration & dosage/immunology
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Immunophenotyping
MH  - Kidney Diseases/immunology/pathology/*prevention & control
MH  - Lupus Erythematosus, Systemic/immunology/pathology/*prevention & control
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Proteinuria/etiology
MH  - Rats
MH  - Rats, Wistar
MH  - T-Lymphocytes/metabolism
MH  - Trypanosoma/immunology
MH  - Vaccines, DNA/*administration & dosage
EDAT- 2011/09/22 06:00
MHDA- 2012/01/13 06:00
CRDT- 2011/09/22 06:00
PHST- 2011/09/22 06:00 [entrez]
PHST- 2011/09/22 06:00 [pubmed]
PHST- 2012/01/13 06:00 [medline]
AID - ker231 [pii]
AID - 10.1093/rheumatology/ker231 [doi]
PST - ppublish
SO  - Rheumatology (Oxford). 2011 Dec;50(12):2187-96. doi: 10.1093/rheumatology/ker231. 
      Epub 2011 Sep 20.