PMID- 21937595
OWN - NLM
STAT- MEDLINE
DCOM- 20111128
LR  - 20110922
IS  - 1477-9129 (Electronic)
IS  - 0950-1991 (Linking)
VI  - 138
IP  - 20
DP  - 2011 Oct
TI  - Cell shape and Wnt signaling redundantly control the division axis of C. elegans 
      epithelial stem cells.
PG  - 4375-85
LID - 10.1242/dev.066431 [doi]
AB  - Tissue-specific stem cells combine proliferative and asymmetric divisions to 
      balance self-renewal with differentiation. Tight regulation of the orientation 
      and plane of cell division is crucial in this process. Here, we study the 
      reproducible pattern of anterior-posterior-oriented stem cell-like divisions in 
      the Caenorhabditis elegans seam epithelium. In a genetic screen, we identified an 
      alg-1 Argonaute mutant with additional and abnormally oriented seam cell 
      divisions. ALG-1 is the main subunit of the microRNA-induced silencing complex 
      (miRISC) and was previously shown to regulate the timing of postembryonic 
      development. Time-lapse fluorescence microscopy of developing larvae revealed 
      that reduced alg-1 function successively interferes with Wnt signaling, cell 
      adhesion, cell shape and the orientation and timing of seam cell division. We 
      found that Wnt inactivation, through mig-14 Wntless mutation, disrupts tissue 
      polarity but not anterior-posterior division. However, combined Wnt inhibition 
      and cell shape alteration resulted in disordered orientation of seam cell 
      division, similar to the alg-1 mutant. Our findings reveal additional 
      alg-1-regulated processes, uncover a previously unknown function of Wnt ligands 
      in seam tissue polarity, and show that Wnt signaling and geometric cues 
      redundantly control the seam cell division axis.
FAU - Wildwater, Marjolein
AU  - Wildwater M
AD  - Department of Developmental Biology, Utrecht University, Padualaan 8, 3584 CH 
      Utrecht, The Netherlands. wildwaterm@gmail.com
FAU - Sander, Nicholas
AU  - Sander N
FAU - de Vreede, Geert
AU  - de Vreede G
FAU - van den Heuvel, Sander
AU  - van den Heuvel S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (ALG-1 protein, C elegans)
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (DNA, Helminth)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Wnt Proteins)
RN  - 0 (beta Catenin)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Caenorhabditis elegans/*cytology/genetics/growth & development/*metabolism
MH  - Caenorhabditis elegans Proteins/genetics/*metabolism
MH  - Cell Division
MH  - Cell Polarity
MH  - Cell Shape
MH  - DNA, Helminth/genetics
MH  - Epithelial Cells/cytology/metabolism
MH  - Gene Expression Regulation, Developmental
MH  - Genes, Helminth
MH  - Mutation
MH  - RNA-Binding Proteins/genetics/metabolism
MH  - Signal Transduction
MH  - Stem Cells/*cytology/*metabolism
MH  - Wnt Proteins/*metabolism
MH  - beta Catenin/metabolism
EDAT- 2011/09/23 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/09/23 06:00
PHST- 2011/09/23 06:00 [entrez]
PHST- 2011/09/23 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - 138/20/4375 [pii]
AID - 10.1242/dev.066431 [doi]
PST - ppublish
SO  - Development. 2011 Oct;138(20):4375-85. doi: 10.1242/dev.066431.