PMID- 21939530
OWN - NLM
STAT- MEDLINE
DCOM- 20120822
LR  - 20211020
IS  - 1466-609X (Electronic)
IS  - 1364-8535 (Print)
IS  - 1364-8535 (Linking)
VI  - 15
IP  - 5
DP  - 2011
TI  - Serial increase of IL-12 response and human leukocyte antigen-DR expression in 
      severe sepsis survivors.
PG  - R224
LID - 10.1186/cc10464 [doi]
AB  - INTRODUCTION: Sepsis-induced immunosuppression may result in death. The 
      mechanisms of immune suppression include loss of macrophage and monocyte 
      expression of the major histocompatibility complex, increased anti-inflammatory 
      cytokine expression and decreased expression of proinflammatory cytokines. In 
      this study, we sought to determine the mechanisms of immune suppression in severe 
      sepsis by repeated detection. METHODS: We designed this prospective observational 
      study to measure monocyte human leukocyte antigen (HLA)-DR expression, plasma 
      cytokine levels and cytokine responses on days 1 and 7 in stimulated peripheral 
      blood mononuclear cells (PBMCs) of healthy controls and patients with severe 
      sepsis. RESULTS: Of the 35 enrolled patients, 23 survived for 28 days and 12 
      died, 6 of whom died within 7 days. Plasma levels of IL-1beta, IL-6, IL-10, IL-17, 
      transforming growth factor (TGF)-beta1 and TNF-alpha were higher, but plasma IL-12 level 
      was lower in septic patients than those in controls. Day 1 plasma levels of 
      IL-1beta, IL-6, IL-10 and TGF-beta1 in nonsurvivors were higher than those in 
      survivors. Day 7 plasma IL-10 levels in nonsurvivors were higher than in 
      survivors. IL-1beta response was higher, but IL-12 and TNF-alpha responses were lower in 
      septic patients than in controls. Day 1 IL-6 response was lower, but day 1 TGF-beta1 
      response was higher in nonsurvivors than in survivors. Plasma IL-6 and IL-10 
      levels were decreased in survivors after 6 days. IL-6 response was decreased in 
      survivors after 6 days, but IL-12 response was increased. Monocyte percentage was 
      higher, but positive HLA-DR percentage in monocytes and mean fluorescence 
      intensity (MFI) of HLA-DR were lower in septic patients than in controls. MFI of 
      HLA-DR was increased in survivors after 6 days. CONCLUSIONS: Monocyte HLA-DR 
      expression and IL-12 response from PBMCs are restored in patients who survive 
      severe sepsis.
FAU - Wu, Huang-Pin
AU  - Wu HP
AD  - Division of Pulmonary, Critical Care and Sleep Medicine, Chang Gung Memorial 
      Hospital, 222 Mai-Chin Road, Keelung, 204, Taiwan. whanpyng@cgmh.org.tw
FAU - Shih, Chi-Chung
AU  - Shih CC
FAU - Lin, Chun-Yao
AU  - Lin CY
FAU - Hua, Chung-Ching
AU  - Hua CC
FAU - Chuang, Duen-Yau
AU  - Chuang DY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110922
PL  - England
TA  - Crit Care
JT  - Critical care (London, England)
JID - 9801902
RN  - 0 (Biomarkers)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
CIN - Crit Care. 2012;16(1):410. PMID: 22316135
MH  - Aged
MH  - Biomarkers/metabolism
MH  - Female
MH  - HLA-DR Antigens/*metabolism
MH  - Humans
MH  - Interleukin-12/*blood
MH  - Leukocytes, Mononuclear/immunology
MH  - Male
MH  - Prospective Studies
MH  - Sepsis/*immunology/mortality
MH  - *Survivors
MH  - Time Factors
MH  - Transforming Growth Factor beta1/blood
MH  - Tumor Necrosis Factor-alpha/blood
PMC - PMC3334770
EDAT- 2011/09/24 06:00
MHDA- 2012/08/23 06:00
PMCR- 2011/09/22
CRDT- 2011/09/24 06:00
PHST- 2011/04/25 00:00 [received]
PHST- 2011/08/05 00:00 [revised]
PHST- 2011/09/22 00:00 [accepted]
PHST- 2011/09/24 06:00 [entrez]
PHST- 2011/09/24 06:00 [pubmed]
PHST- 2012/08/23 06:00 [medline]
PHST- 2011/09/22 00:00 [pmc-release]
AID - cc10464 [pii]
AID - 10.1186/cc10464 [doi]
PST - ppublish
SO  - Crit Care. 2011;15(5):R224. doi: 10.1186/cc10464. Epub 2011 Sep 22.