PMID- 21939635
OWN - NLM
STAT- MEDLINE
DCOM- 20120510
LR  - 20211020
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Electronic)
IS  - 0006-3002 (Linking)
VI  - 1817
IP  - 4
DP  - 2012 Apr
TI  - Design of photoactive ruthenium complexes to study electron transfer and proton 
      pumping in cytochrome oxidase.
PG  - 567-74
LID - 10.1016/j.bbabio.2011.08.012 [doi]
AB  - This review describes the development and application of photoactive ruthenium 
      complexes to study electron transfer and proton pumping reactions in cytochrome c 
      oxidase (CcO). CcO uses four electrons from Cc to reduce O(2) to two waters, and 
      pumps four protons across the membrane. The electron transfer reactions in 
      cytochrome oxidase are very rapid, and cannot be resolved by stopped-flow mixing 
      techniques. Methods have been developed to covalently attach a photoactive 
      tris(bipyridine)ruthenium group [Ru(II)] to Cc to form Ru-39-Cc. Photoexcitation 
      of Ru(II) to the excited state Ru(II*), a strong reductant, leads to rapid 
      electron transfer to the ferric heme group in Cc, followed by electron transfer 
      to Cu(A) in CcO with a rate constant of 60,000s(-1). Ruthenium kinetics and 
      mutagenesis studies have been used to define the domain for the interaction 
      between Cc and CcO. New ruthenium dimers have also been developed to rapidly 
      inject electrons into Cu(A) of CcO with yields as high as 60%, allowing 
      measurement of the kinetics of electron transfer and proton release at each step 
      in the oxygen reduction mechanism.
CI  - Copyright A(c) 2011 Elsevier B.V. All rights reserved.
FAU - Durham, Bill
AU  - Durham B
AD  - Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, 
      AR, USA. bdurham@uark.edu
FAU - Millett, Francis
AU  - Millett F
LA  - eng
GR  - 1P30RR031154-01/RR/NCRR NIH HHS/United States
GR  - F32 GM020488/GM/NIGMS NIH HHS/United States
GR  - R01 GM020488-36/GM/NIGMS NIH HHS/United States
GR  - GM20488/GM/NIGMS NIH HHS/United States
GR  - R01 GM020488/GM/NIGMS NIH HHS/United States
GR  - P30 RR031154-02/RR/NCRR NIH HHS/United States
GR  - P30 GM103450/GM/NIGMS NIH HHS/United States
GR  - P30 RR031154/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Review
DEP - 20110910
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Organometallic Compounds)
RN  - 0 (Protons)
RN  - 7UI0TKC3U5 (Ruthenium)
RN  - 9007-43-6 (Cytochromes c)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Animals
MH  - Biological Transport/radiation effects
MH  - Cattle
MH  - Cytochromes c/chemistry/metabolism
MH  - Electron Transport/radiation effects
MH  - Electron Transport Complex IV/*chemistry/metabolism
MH  - Models, Molecular
MH  - Organometallic Compounds/*chemistry/metabolism
MH  - Oxidation-Reduction/radiation effects
MH  - *Protons
MH  - Ruthenium/*chemistry/metabolism
PMC - PMC3246025
MID - NIHMS325362
EDAT- 2011/09/24 06:00
MHDA- 2012/05/11 06:00
PMCR- 2013/04/01
CRDT- 2011/09/24 06:00
PHST- 2011/06/16 00:00 [received]
PHST- 2011/08/25 00:00 [revised]
PHST- 2011/08/26 00:00 [accepted]
PHST- 2011/09/24 06:00 [entrez]
PHST- 2011/09/24 06:00 [pubmed]
PHST- 2012/05/11 06:00 [medline]
PHST- 2013/04/01 00:00 [pmc-release]
AID - S0005-2728(11)00211-8 [pii]
AID - 10.1016/j.bbabio.2011.08.012 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2012 Apr;1817(4):567-74. doi: 10.1016/j.bbabio.2011.08.012. 
      Epub 2011 Sep 10.