PMID- 21942394 OWN - NLM STAT- MEDLINE DCOM- 20120202 LR - 20171116 IS - 1532-4117 (Electronic) IS - 1093-4529 (Linking) VI - 46 IP - 12 DP - 2011 TI - Feasibility of sunflower oil cake degradation with three different anaerobic consortia. PG - 1409-16 LID - 10.1080/10934529.2011.607032 [doi] AB - Sunflower oil cake (SuOC) is the solid by-product from the sunflower oil extraction process and an important pollutant waste because of its high organic content. For the anaerobic digestion of SuOC three different industrial reactors were compared as inoculum sources. This was done using a biochemical methane production (BMP) test. Inoculum I was a granular biomass from an industrial reactor treating soft-drink wastewaters. Inoculum II was a flocculent biomass from a full-scale reactor treating biosolids generated in an urban wastewater treatment plant. Inoculum III was a granular biomass from an industrial reactor treating brewery wastes. The highest kinetic constant for methane production was achieved using inoculum II. The inoculum sources were analyzed through PCR amplification of 16S rRNA genes and fingerprinting before (t = 0) and after the BMP test (t = 12 days). No significant differences were found in the bacterial community fingerprints between the beginning and the end of the experiments. The bacterial and archaeal communities of inoculum II were further analyzed. The main bacteria found in this inoculum belong to Alphaproteobacteria and Chloroflexi. Of the Archaea detected, Methanomicrobiales and Methanosarcinales made up practically the whole archaeal community. The results showed the importance of selecting an appropriate inoculum in short term processes due to the fact that the major microbial constituents in the initial consortia remained stable throughout anaerobic digestion. FAU - Rincon, Barbara AU - Rincon B AD - Instituto de la Grasa, CSIC, Sevilla, Spain. brlloren@cica.es FAU - Portillo, Maria Del Carmen AU - Portillo Mdel C FAU - Gonzalez, Juan M AU - Gonzalez JM FAU - Fernandez-Cegri, Victoria AU - Fernandez-Cegri V FAU - De La Rubia, Maria Angeles AU - De La Rubia MA FAU - Borja, Rafael AU - Borja R LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - J Environ Sci Health A Tox Hazard Subst Environ Eng JT - Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering JID - 9812551 RN - 0 (Plant Oils) RN - 0 (RNA, Ribosomal, 16S) RN - 0 (Sunflower Oil) RN - OP0UW79H66 (Methane) SB - IM MH - Alphaproteobacteria/genetics/metabolism MH - Anaerobiosis MH - Archaea/genetics/metabolism MH - Bacteria/genetics/metabolism MH - Biodegradation, Environmental MH - Bioreactors/*microbiology MH - Chloroflexi/genetics/metabolism MH - Methane/metabolism MH - Methanomicrobiales/genetics/metabolism MH - Methanosarcinales/metabolism MH - Plant Oils/*metabolism MH - RNA, Ribosomal, 16S/genetics MH - Sunflower Oil MH - Waste Disposal, Fluid/*methods EDAT- 2011/09/29 06:00 MHDA- 2012/02/03 06:00 CRDT- 2011/09/28 06:00 PHST- 2011/09/28 06:00 [entrez] PHST- 2011/09/29 06:00 [pubmed] PHST- 2012/02/03 06:00 [medline] AID - 10.1080/10934529.2011.607032 [doi] PST - ppublish SO - J Environ Sci Health A Tox Hazard Subst Environ Eng. 2011;46(12):1409-16. doi: 10.1080/10934529.2011.607032.