PMID- 21942443
OWN - NLM
STAT- MEDLINE
DCOM- 20120618
LR  - 20120416
IS  - 1557-7430 (Electronic)
IS  - 1044-5498 (Linking)
VI  - 31
IP  - 4
DP  - 2012 Apr
TI  - Interaction of MTHFR 1298C with ACE D allele augments the risk of diabetic 
      nephropathy in Western Iran.
PG  - 553-9
LID - 10.1089/dna.2011.1364 [doi]
AB  - The aim of the current study was to examine the influence of interaction between 
      polymorphisms of methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C 
      with angiotensin converting enzyme insertion/deletion (ACE I/D) polymorphism on 
      the risk of diabetic nephropathy (DN). In a case control study using polymerase 
      chain reaction (PCR)- and PCR-restriction fragment length polymorphism (RFLP), 
      the presence of three polymorphisms in 140 patients with type 2 diabetes mellitus 
      (T2DM) with nephropathy including patients with micro- and macro-albuminuria and 
      72 patients with normoalbuminuria from Western Iran were investigated. In the 
      presence of both MTHFR 677 T and ACE D alleles, there was a trend toward 
      increased risk of DN 2.68-fold (p=0.054). The possession of both MTHFR 677 T and 
      ACE D alleles increased the risk of macro-albuminuria four times (p=0.035). The 
      concomitant presence of both MTHFR 1298 C and ACE D alleles increased the risk of 
      macro-albuminuria 7.8-fold (p=0.012). In addition, the risk of progression from 
      micro- to macro-albuminuria in the presence of both alleles tended to be 
      increased (4.1-fold, p=0.09). Our study for the first time demonstrated a 
      synergistic effect between ACE I/D with either MTHFR C677T or A1298C polymorphism 
      on the increased risk of DN among patients with T2DM. We found that MTHFR 1298 C 
      strongly interacts with the ACE D allele and augments the risk of DN in our 
      population.
FAU - Rahimi, Zohreh
AU  - Rahimi Z
AD  - Medical Biology Research Center, Kermanshah University of Medical Sciences, 
      Kermanshah, Iran. zrahimi@kums.ac.ir
FAU - Hasanvand, Ali
AU  - Hasanvand A
FAU - Felehgari, Vahid
AU  - Felehgari V
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110923
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (DNA Primers)
RN  - EC 1.5.1.20 (Methylenetetrahydrofolate Reductase (NADPH2))
RN  - EC 3.4.15.1 (ACE protein, human)
RN  - EC 3.4.15.1 (Peptidyl-Dipeptidase A)
SB  - IM
MH  - Albuminuria/epidemiology/*genetics
MH  - DNA Primers/genetics
MH  - Diabetes Mellitus, Type 2/*complications
MH  - Diabetic Nephropathies/epidemiology/*genetics
MH  - Female
MH  - Gene Frequency
MH  - Genetic Association Studies
MH  - Genetic Predisposition to Disease/*genetics
MH  - Genotype
MH  - Humans
MH  - Iran/epidemiology
MH  - Male
MH  - Methylenetetrahydrofolate Reductase (NADPH2)/*genetics
MH  - Middle Aged
MH  - Odds Ratio
MH  - Peptidyl-Dipeptidase A/*genetics
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Genetic/*genetics
MH  - Polymorphism, Restriction Fragment Length
MH  - Risk Factors
EDAT- 2011/09/29 06:00
MHDA- 2012/06/19 06:00
CRDT- 2011/09/28 06:00
PHST- 2011/09/28 06:00 [entrez]
PHST- 2011/09/29 06:00 [pubmed]
PHST- 2012/06/19 06:00 [medline]
AID - 10.1089/dna.2011.1364 [doi]
PST - ppublish
SO  - DNA Cell Biol. 2012 Apr;31(4):553-9. doi: 10.1089/dna.2011.1364. Epub 2011 Sep 
      23.