PMID- 21944540
OWN - NLM
STAT- MEDLINE
DCOM- 20120301
LR  - 20111107
IS  - 1873-2232 (Electronic)
IS  - 0378-4320 (Linking)
VI  - 128
IP  - 1-4
DP  - 2011 Oct
TI  - Association study and expression analysis of porcine ESR1 as a candidate gene for 
      boar fertility and sperm quality.
PG  - 11-21
LID - 10.1016/j.anireprosci.2011.08.008 [doi]
AB  - Male fertility is impaired through the lack of ESR1 (Estrogen Receptor 1) but 
      little is known about the ESR1 roles in boar spermatogenesis and fertility. 
      Therefore, this research was aimed at investigating the association with sperm 
      quality and boar fertility traits in a total of 300 boars both from purebred 
      Pietrain and Pietrain x Hampshire crosses. A SNP in coding region of ESR1g.672C>T 
      in exon 1 was associated with sperm motility (P<0.05) and plasma droplet rate 
      (P<0.01) while the polymorphism in non-coding region of ESR1g.35756T>C in inton 1 
      was associated with non-return rate (P<0.05). Furthermore, to analyse the mRNA 
      and protein expression of ESR1 in boar reproductive tissues, a total of six boars 
      were divided into two groups [Group I (G-I) and Group II (G-II)], where G-I had 
      relatively better sperm quality. ESR1 expression was higher in tissues collected 
      from G-I boars than those of collected from G-II boars, and the difference in 
      mRNA expression was significant (P<0.01) in head of epididymis. The ESR1 protein 
      expression results from western blot coincided with the results of qRT-PCR. The 
      ESR1 protein localization observed a strong staining in the cytoplasm of Sertoli 
      cell in the testis, in the epithelial cells in head and tail of epididymis, in 
      smooth muscle in tail of epididymis, and in the post acrosomal region and tail of 
      the spermatozoa. These results will improve the understanding of the functions of 
      the ESR1 in spermatogenesis within the reproductive tract and will shed light on 
      ESR1 as a candidate in the selection of boar with good sperm quality and 
      fertility.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Gunawan, Asep
AU  - Gunawan A
AD  - Institute of Animal Science, Animal Breeding and Husbandry Group, University of 
      Bonn, Bonn, Germany. agun@itw.uni-bonn.de
FAU - Kaewmala, Kanokwan
AU  - Kaewmala K
FAU - Uddin, Muhammad Jasim
AU  - Uddin MJ
FAU - Cinar, Mehmet Ulas
AU  - Cinar MU
FAU - Tesfaye, Dawit
AU  - Tesfaye D
FAU - Phatsara, Chirawath
AU  - Phatsara C
FAU - Tholen, Ernst
AU  - Tholen E
FAU - Looft, Christian
AU  - Looft C
FAU - Schellander, Karl
AU  - Schellander K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110906
PL  - Netherlands
TA  - Anim Reprod Sci
JT  - Animal reproduction science
JID - 7807205
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Estrogen Receptor alpha/genetics/*metabolism
MH  - Fertility/*physiology
MH  - Genitalia, Male/metabolism
MH  - Male
MH  - Polymorphism, Genetic
MH  - Protein Transport
MH  - RNA, Messenger/genetics/metabolism
MH  - Spermatogenesis/physiology
MH  - Spermatozoa/*physiology
MH  - Swine/*physiology
EDAT- 2011/09/29 06:00
MHDA- 2012/03/02 06:00
CRDT- 2011/09/28 06:00
PHST- 2011/05/13 00:00 [received]
PHST- 2011/08/12 00:00 [revised]
PHST- 2011/08/24 00:00 [accepted]
PHST- 2011/09/28 06:00 [entrez]
PHST- 2011/09/29 06:00 [pubmed]
PHST- 2012/03/02 06:00 [medline]
AID - S0378-4320(11)00223-5 [pii]
AID - 10.1016/j.anireprosci.2011.08.008 [doi]
PST - ppublish
SO  - Anim Reprod Sci. 2011 Oct;128(1-4):11-21. doi: 10.1016/j.anireprosci.2011.08.008. 
      Epub 2011 Sep 6.