PMID- 21944563
OWN - NLM
STAT- MEDLINE
DCOM- 20111202
LR  - 20211020
IS  - 1090-2163 (Electronic)
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 271
IP  - 2
DP  - 2011
TI  - Mountain cedar pollen induces IgE-independent mast cell degranulation, IL-4 
      production, and intracellular reactive oxygen species generation.
PG  - 488-95
LID - 10.1016/j.cellimm.2011.08.019 [doi]
AB  - Cedar pollens cause severe allergic disease throughout the world. We have 
      previously characterized allergenic pollen glycoproteins from mountain cedar 
      (Juniperus ashei) that bind to allergen-specific immunoglobulin E (IgE). In the 
      present report, we investigated an alternative pathway of mast cell activation by 
      mountain cedar pollen extract through IgE-independent mechanisms. We show that 
      mountain cedar pollen directly induces mast cell serotonin and IL-4 release and 
      enhances release induced by IgE cross-linking. Concomitant with mediator release, 
      high levels of intracellular reactive oxygen species (ROS) were generated, and 
      both ROS and serotonin release were inhibited by anti-oxidants. These findings 
      suggest that alternative mechanisms exist whereby pollen exposure enhances 
      allergic inflammatory mediator release through mechanisms that involve ROS. These 
      mechanisms have the potential for enhancing the allergenic potency of pollens.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Endo, Shuichiro
AU  - Endo S
AD  - Department of Otolaryngology-Head and Neck Surgery, University of Yamanashi, 
      Chuo, Yamanashi, Japan.
FAU - Hochman, Daniel J
AU  - Hochman DJ
FAU - Midoro-Horiuti, Terumi
AU  - Midoro-Horiuti T
FAU - Goldblum, Randall M
AU  - Goldblum RM
FAU - Brooks, Edward G
AU  - Brooks EG
LA  - eng
GR  - T32 ES007254-10A1/ES/NIEHS NIH HHS/United States
GR  - P30 ES006676-09/ES/NIEHS NIH HHS/United States
GR  - T32-07524/PHS HHS/United States
GR  - T32 ES007254/ES/NIEHS NIH HHS/United States
GR  - P30 ES006676/ES/NIEHS NIH HHS/United States
GR  - ES06676/ES/NIEHS NIH HHS/United States
GR  - P30 HD 27841/HD/NICHD NIH HHS/United States
GR  - P30 HD027841-07/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110831
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Antioxidants)
RN  - 0 (Biogenic Amines)
RN  - 0 (IL4 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 207137-56-2 (Interleukin-4)
RN  - 333DO1RDJY (Serotonin)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Biogenic Amines/biosynthesis
MH  - Cell Degranulation/drug effects/immunology
MH  - Cell Line
MH  - Humans
MH  - Immunoglobulin E/metabolism
MH  - Interleukin-4/*biosynthesis/genetics
MH  - Juniperus/*immunology
MH  - Mast Cells/drug effects/*immunology/physiology
MH  - Pollen/*adverse effects/immunology
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Reactive Oxygen Species/metabolism
MH  - Rhinitis, Allergic, Seasonal/etiology/immunology
MH  - Serotonin/biosynthesis
MH  - Up-Regulation
PMC - PMC3195927
MID - NIHMS321922
EDAT- 2011/09/29 06:00
MHDA- 2011/12/13 00:00
PMCR- 2012/08/31
CRDT- 2011/09/28 06:00
PHST- 2009/10/20 00:00 [received]
PHST- 2011/07/26 00:00 [revised]
PHST- 2011/08/24 00:00 [accepted]
PHST- 2011/09/28 06:00 [entrez]
PHST- 2011/09/29 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2012/08/31 00:00 [pmc-release]
AID - S0008-8749(11)00232-2 [pii]
AID - 10.1016/j.cellimm.2011.08.019 [doi]
PST - ppublish
SO  - Cell Immunol. 2011;271(2):488-95. doi: 10.1016/j.cellimm.2011.08.019. Epub 2011 
      Aug 31.