PMID- 21945121
OWN - NLM
STAT- MEDLINE
DCOM- 20120315
LR  - 20220331
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 56
IP  - 3
DP  - 2011 Dec
TI  - TH1 and TH17 interactions in untreated inflamed mucosa of inflammatory bowel 
      disease, and their potential to mediate the inflammation.
PG  - 633-40
LID - 10.1016/j.cyto.2011.08.036 [doi]
AB  - BACKGROUND: Crohn's disease (CD) and ulcerative colitis (UC) have been associated 
      with a T helper1 (TH1) and a TH2 cytokine profile, respectively. Recently, a TH17 
      lineage has been introduced, but their role in the inflammation of CD and UC is 
      not fully understood. AIM: To characterize the cytokines directing the TH17 cells 
      and their interactions with TH1 cells in the mucosa of untreated patients with CD 
      and UC. METHOD: Seventy-nine patients with untreated UC, 32 patients with 
      untreated CD and 23 controls with no signs of colon disease were included in the 
      study. Clinical indices for ulcerative colitis (UCDAI) and Crohn's disease (CDAI) 
      were assessed. Biopsies for measurements of interleukin (IL)-17A, IL-23, IL-6, 
      transforming growth factor-beta (TGF-beta), interferon-gamma (IFN-gamma), mRNA levels as 
      well as immunohistochemical (IHC) analyses were performed. RESULTS: The gene 
      expression for all cytokines in UC and for all cytokines except for TGF-beta in CD 
      were significantly increased compared with the controls. The immunohistochemical 
      analysis showed significantly increased number of IL-17A positive cells in lamina 
      propria and epithelium of both UC and CD compared to controls. The levels of 
      IL-17A and IL-23 mRNA were significantly higher in UC than in CD while the levels 
      of IL-6 were significantly higher in CD compared with UC. The levels of IL-17A, 
      IL-6 and IL-23 mRNA were associated with the disease activity score in both UC 
      and CD. IFN-gamma was associated with the disease activity in UC, but did not reach 
      significant level in CD. CONCLUSION: Increased levels of IL-17A and IL-23 were 
      found in both UC and CD compared to controls. Association to the grade of 
      inflammation and clinical activity was also observed. IL-17A and IL-23 were 
      significantly higher in UC than in CD. TH1 and TH17 cytokines seem to act 
      synergistically in inflammatory bowel disease (IBD) with no apparent polarization 
      between UC and CD.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Olsen, Trine
AU  - Olsen T
AD  - Laboratory of Gastroenterology, Institute of Clinical Medicine, University of 
      Tromso, and Department of Medical Gastroenterology, University Hospital of 
      Northern Norway, 9037 Tromso, Norway. trine.olsen@fagmed.uit.no
FAU - Rismo, Renathe
AU  - Rismo R
FAU - Cui, Guanglin
AU  - Cui G
FAU - Goll, Rasmus
AU  - Goll R
FAU - Christiansen, Ingrid
AU  - Christiansen I
FAU - Florholmen, Jon
AU  - Florholmen J
LA  - eng
PT  - Journal Article
DEP - 20110925
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Case-Control Studies
MH  - Cell Communication/*immunology
MH  - Cytokines/genetics/metabolism
MH  - Demography
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - Immunohistochemistry
MH  - Inflammation/complications/immunology/*pathology
MH  - Inflammatory Bowel Diseases/diagnosis/*immunology/*pathology/therapy
MH  - Intestinal Mucosa/*pathology
MH  - Male
MH  - Middle Aged
MH  - RNA, Messenger/genetics/metabolism
MH  - Th1 Cells/*immunology/pathology
MH  - Th17 Cells/*immunology/pathology
MH  - Young Adult
EDAT- 2011/09/29 06:00
MHDA- 2012/03/16 06:00
CRDT- 2011/09/28 06:00
PHST- 2010/11/11 00:00 [received]
PHST- 2011/08/23 00:00 [revised]
PHST- 2011/08/25 00:00 [accepted]
PHST- 2011/09/28 06:00 [entrez]
PHST- 2011/09/29 06:00 [pubmed]
PHST- 2012/03/16 06:00 [medline]
AID - S1043-4666(11)00716-2 [pii]
AID - 10.1016/j.cyto.2011.08.036 [doi]
PST - ppublish
SO  - Cytokine. 2011 Dec;56(3):633-40. doi: 10.1016/j.cyto.2011.08.036. Epub 2011 Sep 
      25.