PMID- 21945219
OWN - NLM
STAT- MEDLINE
DCOM- 20120208
LR  - 20191210
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 178
IP  - 1-2
DP  - 2011 Dec
TI  - Rapid and sensitive detection of mud crab Scylla serrata reovirus by a reverse 
      transcription loop-mediated isothermal amplification assay.
PG  - 153-60
LID - 10.1016/j.jviromet.2011.09.006 [doi]
AB  - Scylla serrata reovirus (SsRV) is one of the most prevalent viral pathogens of 
      the mud crab (S. serrata). This pathogen is widespread in east China and causes 
      severe economic losses to the nation's mud crab industry. Early detection of this 
      pathogen is necessary for disease control and reduction of economic loss. In the 
      present study, a reverse transcriptase loop-mediated isothermal amplification 
      (RT-LAMP) assay for the rapid and sensitive detection of SsRV was developed and 
      evaluated. The LAMP reaction mix was optimized, as were the reaction temperature 
      (62 degrees C) and the duration of the assay (60min). The sensitivity of the RT-LAMP 
      assay was determined to be 0.8fg SsRV dsRNA, which was 1000-fold higher than that 
      of a one-step reverse transcription polymerase chain reaction (RT-PCR). The 
      RT-LAMP assay also had higher sensitivity than a one-step RT-PCR, as it 
      identified nine more positive cases from 55 mud crabs suspected of having SsRV. 
      No cross-reactivity was found with the DNA/RNA of other tested viruses and 
      SsRV-negative animals. Importantly, the assay can be completed within 60min and 
      is faster than conventional RT-PCR. In summary, the RT-LAMP assay is a simple, 
      cost-effective, sensitive, and specific tool for the rapid detection of SsRV 
      infection.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Chen, Jigang
AU  - Chen J
AD  - College of Biological and Environmental Sciences, Zhejiang Wanli University, No. 
      8, South Qianhu Road, Ningbo 315100, China. genomic@163.com
FAU - Xiong, Juan
AU  - Xiong J
FAU - Cui, Bojing
AU  - Cui B
FAU - Yang, Jifang
AU  - Yang J
FAU - Mao, Zhijuan
AU  - Mao Z
FAU - Li, Wenchen
AU  - Li W
FAU - Chen, Xiaoxuan
AU  - Chen X
FAU - Zheng, Xiaojuan
AU  - Zheng X
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110917
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
SB  - IM
MH  - Animals
MH  - Brachyura/*virology
MH  - China
MH  - Cross Reactions
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Reoviridae/*isolation & purification
MH  - Sensitivity and Specificity
MH  - Temperature
MH  - Time Factors
MH  - Veterinary Medicine/*methods
MH  - Virology/*methods
EDAT- 2011/09/29 06:00
MHDA- 2012/02/09 06:00
CRDT- 2011/09/28 06:00
PHST- 2011/04/12 00:00 [received]
PHST- 2011/09/01 00:00 [revised]
PHST- 2011/09/12 00:00 [accepted]
PHST- 2011/09/28 06:00 [entrez]
PHST- 2011/09/29 06:00 [pubmed]
PHST- 2012/02/09 06:00 [medline]
AID - S0166-0934(11)00381-8 [pii]
AID - 10.1016/j.jviromet.2011.09.006 [doi]
PST - ppublish
SO  - J Virol Methods. 2011 Dec;178(1-2):153-60. doi: 10.1016/j.jviromet.2011.09.006. 
      Epub 2011 Sep 17.