PMID- 21947186
OWN - NLM
STAT- MEDLINE
DCOM- 20120426
LR  - 20211020
IS  - 1432-1262 (Electronic)
IS  - 0179-1958 (Linking)
VI  - 27
IP  - 1
DP  - 2012 Jan
TI  - Down-regulation of HLA-A mRNA in peripheral blood mononuclear cell of colorectal 
      cancer.
PG  - 31-6
LID - 10.1007/s00384-011-1315-4 [doi]
AB  - PURPOSE: It has been demonstrated that the alteration of human leukocyte antigen 
      (HLA) class I expression frequently occurs in colorectal tumor. Previous studies 
      mainly focused on the expression of HLA-A in tumor cells. The expression of HLA-A 
      in peripheral blood mononuclear cells (PBMC) was unknown. To develop a 
      non-invasive diagnostic method for colorectal cancer (CRC), this work 
      investigated the expression of HLA-A mRNA in PBMC in patients with CRC. METHODS: 
      Real-time quantitative RT-PCR was used to study the expression of HLA-A mRNA in 
      PBMC from 48 patients with colorectal cancer, 38 patients with benign colorectal 
      lesions, 20 patients with rheumatoid arthritis, 20 patients with esophageal 
      cancer and 40 healthy individuals. Protein chip was utilized to detect the levels 
      of serum CEA, CA 19-9, and CA 242 in all the cases. Overall results from the two 
      methods were compared. RESULTS: The relative expression of HLA-A mRNA in PBMC was 
      1.11 +/- 0.45 in healthy group, 0.81 +/- 0.42 in benign colorectal lesion group, and 
      0.39 +/- 0.34 in cancer group, respectively. The diagnostic sensitivity of HLA-A 
      mRNA, CEA, CA19-9, and CA242 was 81%, 59%, 61%, and 63%, and their diagnostic 
      specificity was 75%, 64%, 52%, and 67%, respectively. CONCLUSIONS: The expression 
      of HLA-A mRNA in PBMC from colorectal cancer group was significantly lower than 
      those in both benign group and healthy group (P < 0.001). It could be potentially 
      developed as a tumor assistant marker in future.
FAU - Zhu, Ming-Chen
AU  - Zhu MC
AD  - Department of Clinical Laboratory, Jiangsu Cancer Hospital, Nanjing, 210009, 
      Jiangsu Province, China.
FAU - Xu, Yi-Jun
AU  - Xu YJ
FAU - Zou, Xiong
AU  - Zou X
FAU - Yan, Feng
AU  - Yan F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110923
PL  - Germany
TA  - Int J Colorectal Dis
JT  - International journal of colorectal disease
JID - 8607899
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (HLA-A Antigens)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/blood/genetics
MH  - Colorectal Neoplasms/*blood/*genetics/immunology/pathology
MH  - Down-Regulation/*genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - HLA-A Antigens/*blood/*genetics
MH  - Humans
MH  - Leukocytes, Mononuclear/metabolism
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/blood/genetics
MH  - ROC Curve
EDAT- 2011/09/29 06:00
MHDA- 2012/04/27 06:00
CRDT- 2011/09/28 06:00
PHST- 2011/08/30 00:00 [accepted]
PHST- 2011/09/28 06:00 [entrez]
PHST- 2011/09/29 06:00 [pubmed]
PHST- 2012/04/27 06:00 [medline]
AID - 10.1007/s00384-011-1315-4 [doi]
PST - ppublish
SO  - Int J Colorectal Dis. 2012 Jan;27(1):31-6. doi: 10.1007/s00384-011-1315-4. Epub 
      2011 Sep 23.