PMID- 21947608
OWN - NLM
STAT- MEDLINE
DCOM- 20120514
LR  - 20211020
IS  - 1573-4994 (Electronic)
IS  - 1053-0509 (Linking)
VI  - 22
IP  - 1
DP  - 2012 Jan
TI  - A promising CdSe@CdS-quantum dots-cysteine for the determination of trace IgE by 
      solid substrate room temperature phosphorescence immunoassay.
PG  - 419-29
LID - 10.1007/s10895-011-0975-3 [doi]
AB  - The labelling reagent CdSe@CdS-QDs-Cys (QDs-Cys) with the grain diameter of 
      4.5 nm was synthesized by modifying CdSe@CdS quantum dots (QDs) with cysteine 
      (Cys). At the same time, QDs-Cys-Ab(IgE), a phosphorescent quantum dot probe, was 
      developed based on the labelling reaction between -COOH of QDs-Cys and -NH(2) of 
      goat anti human IgE antibody (Ab(IgE)). This probe with excellent 
      biocompatibility and high specificity could not only emit strong and stable room 
      temperature phosphorescence (RTP), but also could carry out specific immunoassay 
      (IA) with immunoglobulin E (IgE), causing the RTP of the system to sharply 
      enhance. Thus, a new solid substrate room temperature phosphorescence immunoassay 
      (SSRTPIA) for the determination of IgE was established. The limit of 
      quantification (LOQ) of the method was 0.12 fg spot(-1), corresponding 
      concentration was 3.0 x 10(-13) g mL(-1) and sampling quantity was 0.40 muL 
      spot(-1). This highly selective, sensitive and accurate SSRTPIA has been applied 
      to determine IgE in biological samples and diagnose diseases, and the results 
      agreed well with those obtained by enzyme-link immunoassay (ELISA). Meanwhile, 
      the mechanisms of QDs-Cys labelling Ab(IgE) and the determination of IgE by 
      SSRTPIA were also discussed.
FAU - Liu, Jia-Ming
AU  - Liu JM
AD  - Department of Chemistry and Environmental Science, Zhangzhou Normal College, 
      Zhangzhou, 363000, People's Republic of China. zzsyliujiaming@163.com
FAU - Lin, Li-ping
AU  - Lin LP
FAU - Liu, Zhen-Bo
AU  - Liu ZB
FAU - Yang, Min-Lan
AU  - Yang ML
FAU - Wang, Xin-xing
AU  - Wang XX
FAU - Zhang, Li-hong
AU  - Zhang LH
FAU - Cui, Ma-lin
AU  - Cui ML
FAU - Jiao, Li
AU  - Jiao L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110927
PL  - Netherlands
TA  - J Fluoresc
JT  - Journal of fluorescence
JID - 9201341
RN  - 0 (Cadmium Compounds)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Membranes, Artificial)
RN  - 0 (Selenium Compounds)
RN  - 0 (Sulfides)
RN  - 057EZR4Z7Q (cadmium sulfide)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - A7F646JC5C (cadmium selenide)
RN  - K848JZ4886 (Cysteine)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Cadmium Compounds/*chemistry
MH  - Calibration
MH  - Cysteine/*chemistry
MH  - Fluorescent Dyes/chemistry
MH  - Humans
MH  - Humidity
MH  - Immunoassay/*methods
MH  - Immunoglobulin E/*analysis/blood
MH  - Membranes, Artificial
MH  - Oxygen/chemistry
MH  - *Quantum Dots
MH  - Selenium Compounds/*chemistry
MH  - Spectrometry, Fluorescence
MH  - Sulfides/*chemistry
MH  - *Temperature
MH  - Time Factors
EDAT- 2011/09/29 06:00
MHDA- 2012/05/15 06:00
CRDT- 2011/09/28 06:00
PHST- 2011/06/04 00:00 [received]
PHST- 2011/09/13 00:00 [accepted]
PHST- 2011/09/28 06:00 [entrez]
PHST- 2011/09/29 06:00 [pubmed]
PHST- 2012/05/15 06:00 [medline]
AID - 10.1007/s10895-011-0975-3 [doi]
PST - ppublish
SO  - J Fluoresc. 2012 Jan;22(1):419-29. doi: 10.1007/s10895-011-0975-3. Epub 2011 Sep 
      27.