PMID- 21947651
OWN - NLM
STAT- MEDLINE
DCOM- 20120928
LR  - 20181201
IS  - 1573-7217 (Electronic)
IS  - 0167-6806 (Linking)
VI  - 133
IP  - 2
DP  - 2012 Jun
TI  - The in vitro and in vivo effects of human umbilical cord mesenchymal stem cells 
      on the growth of breast cancer cells.
PG  - 473-85
LID - 10.1007/s10549-011-1774-x [doi]
AB  - The purpose of the study was to detect the effect and possible mechanism of human 
      umbilical cord mesenchymal stem cells (hUCMSCs) on the in vitro and in vivo 
      growth of stem cells isolated from primary human breast cancer cells and cell 
      lines MDA-MB-231 and MCF-7. Primary human breast cancer cells and MDA-MB-231 and 
      MCF-7 cells were sorted in vitro using flow cytometry, and the ESA+, CD44+, 
      CD24-/low cells were isolated as breast cancer stem cells (CSCs). The inhibitory 
      effect of hUCMSCs on CSCs was examined using the Cell Counting Kit-8 cell 
      proliferation and soft agar colony formation assay. In vivo tumor inhibition was 
      studied using a severe combined immunodeficient xenograft mouse model 
      transplanted with MDA-MB-231 breast CSCs. The expression of phosphoinositide 
      3-kinase (PI3K) and AKT was examined in the xenograft tumors using 
      immunohistochemistry. The number of colonies formed by breast CSCs co-cultured 
      with hUCMSCs at the bottom of soft agar was significantly lower than those formed 
      by the control group (P < 0.01). Compared with the control group, the CSCs 
      co-cultured with hUCMSCs showed a higher number of cells in the G2-M phase (P < 
      0.05) and an increased number of apoptotic cells (P < 0.01). The mice in the 
      medium- and high-concentration hUCMSC treatment groups exhibited clearly reduced 
      tumor volume and tumor weight, compared with the control group (P < 0.01). 
      Compared with the saline group, the xenograft tumor tissues from the mice treated 
      with different concentrations of hUCMSCs showed significantly reduced levels of 
      PI3K and AKT proteins (P < 0.001). In conclusion, hUCMSC significantly inhibited 
      the growth of breast CSCs in vitro and in vivo. The underlying mechanism is 
      likely related to cell cycle arrest, induction of tumor cell apoptosis, and 
      suppressed activities of PI3K and AKT protein kinases.
FAU - Ma, Yi
AU  - Ma Y
AD  - Tianjin Medical University Cancer Hospital, Tianjin, People's Republic of China.
FAU - Hao, Xiaomeng
AU  - Hao X
FAU - Zhang, Sheng
AU  - Zhang S
FAU - Zhang, Jin
AU  - Zhang J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110923
PL  - Netherlands
TA  - Breast Cancer Res Treat
JT  - Breast cancer research and treatment
JID - 8111104
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Breast Neoplasms/*metabolism
MH  - Cell Cycle Checkpoints
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Cell Transformation, Neoplastic/metabolism
MH  - Female
MH  - Fetal Blood/*cytology
MH  - Humans
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Mice
MH  - Mice, Nude
MH  - Mice, SCID
MH  - Neoplastic Stem Cells/metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Xenograft Model Antitumor Assays
EDAT- 2011/09/29 06:00
MHDA- 2012/09/29 06:00
CRDT- 2011/09/28 06:00
PHST- 2011/05/23 00:00 [received]
PHST- 2011/09/08 00:00 [accepted]
PHST- 2011/09/28 06:00 [entrez]
PHST- 2011/09/29 06:00 [pubmed]
PHST- 2012/09/29 06:00 [medline]
AID - 10.1007/s10549-011-1774-x [doi]
PST - ppublish
SO  - Breast Cancer Res Treat. 2012 Jun;133(2):473-85. doi: 10.1007/s10549-011-1774-x. 
      Epub 2011 Sep 23.