PMID- 21948070
OWN - NLM
STAT- MEDLINE
DCOM- 20120126
LR  - 20211020
IS  - 1573-6857 (Electronic)
IS  - 0016-6707 (Linking)
VI  - 139
IP  - 8
DP  - 2011 Aug
TI  - New insights on the origin of B chromosomes in Astyanax scabripinnis obtained by 
      chromosome painting and FISH.
PG  - 1073-81
LID - 10.1007/s10709-011-9611-z [doi]
AB  - Chromosome painting (CP) with a probe of B chromosome obtained by microdissection 
      and fluorescence in situ hybridization (FISH) with probes of As51 satellite DNA, 
      C( o )t-1 DNA, and 18S and 5S rDNA confirmed sharing of some repetitive DNA but 
      not rDNA between A and B chromosomes in the fish Astyanax scabripinnis. Meiotic 
      analysis revealed a pachytene B chromosome bivalent nearly half the size of its 
      mitotic configuration, suggesting a self-pairing of B chromosome arms. Such an 
      isochromosome nature of somatic B chromosome was further evidenced by CP and 
      FISH. All the findings obtained suggest (i) intraspecific origin of B chromosome, 
      and (ii) evolutionary enrichment of repetitive DNA classes, especially those 
      contained in the C( o )t-1 and the As51 probes, in B chromosome. However, the 
      precise origin of B chromosome in the present species remains to be elucidated by 
      further molecular cytogenetic analysis because of painting of some A chromosome 
      regions with the B chromosome-derived probe.
FAU - Vicari, Marcelo Ricardo
AU  - Vicari MR
AD  - Departamento de Biologia Estrutural, Molecular e Genetica, Universidade Estadual 
      de Ponta Grossa, Av. Carlos Cavalcanti, 4748, Ponta Grossa, PR 84030-900, Brazil.
FAU - de Mello Pistune, Helena Flavia
AU  - de Mello Pistune HF
FAU - Castro, Jonathan Pena
AU  - Castro JP
FAU - de Almeida, Mara Cristina
AU  - de Almeida MC
FAU - Bertollo, Luiz Antonio Carlos
AU  - Bertollo LA
FAU - Moreira-Filho, Orlando
AU  - Moreira-Filho O
FAU - Camacho, Juan Pedro M
AU  - Camacho JP
FAU - Artoni, Roberto Ferreira
AU  - Artoni RF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110927
PL  - Netherlands
TA  - Genetica
JT  - Genetica
JID - 0370740
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Characidae/*genetics
MH  - *Chromosome Painting
MH  - Chromosomes/*genetics
MH  - DNA/genetics
MH  - Female
MH  - Male
MH  - Meiosis/genetics
MH  - Metaphase/genetics
MH  - Microdissection
MH  - Repetitive Sequences, Nucleic Acid/genetics
EDAT- 2011/09/29 06:00
MHDA- 2012/01/27 06:00
CRDT- 2011/09/28 06:00
PHST- 2011/06/11 00:00 [received]
PHST- 2011/09/19 00:00 [accepted]
PHST- 2011/09/28 06:00 [entrez]
PHST- 2011/09/29 06:00 [pubmed]
PHST- 2012/01/27 06:00 [medline]
AID - 10.1007/s10709-011-9611-z [doi]
PST - ppublish
SO  - Genetica. 2011 Aug;139(8):1073-81. doi: 10.1007/s10709-011-9611-z. Epub 2011 Sep 
      27.