PMID- 21948751
OWN - NLM
STAT- MEDLINE
DCOM- 20120302
LR  - 20171116
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 10
IP  - 3
DP  - 2011 Aug 26
TI  - Primed in situ labeling for detecting single-copy genes.
PG  - 1884-90
LID - 10.4238/vol10-3gmr1391 [doi]
AB  - In order to analyze male sterility caused by deletion of SRY and DAZ, we examined 
      the accuracy and cost-effectiveness of a modified primed in situ labeling (PRINS) 
      technique for detection of single-copy genes. Peripheral blood samples were 
      collected from 50 healthy men; medium-term cultured lymphocytes from these 
      samples were suspended in fixative solution and then spread on clean slides. We 
      used four primers homologous to unique regions of the SRY and DAZ regions of the 
      human Y-chromosome and incorporated reagents to increase polymerase specificity 
      and to enhance the hybridization signal. PRINS of SRY and DAZ gave bands at 
      Yp11.3 and Yq11.2, respectively, in all 50 metaphase spreads. The PRINS SRY 
      signals were as distinct as those obtained using traditional fluorescence in situ 
      hybridization (FISH). This new method is ideal for rapid localization of 
      single-copy genes or small DNA segments, making PRINS a cost-effective 
      alternative to FISH. Further enhancement of PRINS to increase its speed of 
      implementation may lead to its wide use in the field of medical genetics.
FAU - Gao, J-L
AU  - Gao JL
AD  - Key Laboratory of Bio-Resources and Eco-Environment, Institute of Medical 
      Genetics, College of Life Science, Sichuan University, Ministry of Education, 
      Chengdu, China.
FAU - Nie, Y
AU  - Nie Y
FAU - Ding, X-P
AU  - Ding XP
LA  - eng
PT  - Journal Article
DEP - 20110826
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (DAZ1 protein, human)
RN  - 0 (DNA Primers)
RN  - 0 (Deleted in Azoospermia 1 Protein)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (SRY protein, human)
RN  - 0 (Sex-Determining Region Y Protein)
SB  - IM
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Y/genetics
MH  - DNA Primers
MH  - Deleted in Azoospermia 1 Protein
MH  - Gene Dosage
MH  - *Genes, sry
MH  - Gonadal Dysgenesis/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Infertility, Male/*genetics
MH  - Lymphocytes
MH  - Male
MH  - Polymerase Chain Reaction/methods
MH  - Primed In Situ Labeling/*methods
MH  - RNA-Binding Proteins/*genetics
MH  - Sex-Determining Region Y Protein/*genetics
MH  - Spermatozoa/cytology/growth & development
EDAT- 2011/09/29 06:00
MHDA- 2012/03/03 06:00
CRDT- 2011/09/28 06:00
PHST- 2011/09/28 06:00 [entrez]
PHST- 2011/09/29 06:00 [pubmed]
PHST- 2012/03/03 06:00 [medline]
AID - gmr1391 [pii]
AID - 10.4238/vol10-3gmr1391 [doi]
PST - epublish
SO  - Genet Mol Res. 2011 Aug 26;10(3):1884-90. doi: 10.4238/vol10-3gmr1391.