PMID- 21949043
OWN - NLM
STAT- MEDLINE
DCOM- 20120306
LR  - 20181201
IS  - 1537-6613 (Electronic)
IS  - 0022-1899 (Linking)
VI  - 205
IP  - 3
DP  - 2012 Feb 1
TI  - Adoptive transfer of dendritic cells pulsed with Fasciola hepatica antigens and 
      lipopolysaccharides confers protection against fasciolosis in mice.
PG  - 506-14
LID - 10.1093/infdis/jir606 [doi]
AB  - Dendritic cells (DCs) can function as adjuvants able to mediate protection 
      against different pathogens. Given that successful vaccination against Fasciola 
      hepatica is mostly related to the induction of Th1 responses, we studied the 
      potential of DCs loaded with F. hepatica antigens and lipopolysaccharide (LPS) 
      (which promote DCs maturation) as a vaccine against fasciolosis in BALB/c mice. 
      However, only a semimature phenotype was achieved when DCs were simultaneously 
      cultured with an F. hepatica total extract (TE) and LPS. The activation status of 
      TE-loaded DCs was enhanced when these cells were treated with TE 90 minutes 
      before being stimulated with LPS (TE90 DCs). More importantly, a single 
      vaccination of mice with TE90 DCs stimulated a systemic Th1 response and 
      conferred protection against hepatic damage induced by F. hepatica infection. 
      Thus, TE90 DCs may prove to be a useful new tool for vaccination against F. 
      hepatica.
FAU - Falcon, Cristian R
AU  - Falcon CR
AD  - Departamento de Bioquimica Clinica, Centro de Investigaciones en Bioquimica e 
      Inmunologia-Consejo Nacional de Investigaciones Cientificas y Tecnicas, Facultad 
      de Ciencias Quimicas. Universidad Nacional de Cordoba, Argentina.
FAU - Carranza, Franco A
AU  - Carranza FA
FAU - Aoki, Pilar
AU  - Aoki P
FAU - Motran, Claudia C
AU  - Motran CC
FAU - Cervi, Laura
AU  - Cervi L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110923
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Antigens, Helminth)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Adoptive Transfer/*methods
MH  - Animals
MH  - Antigens, Helminth/*immunology
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology
MH  - Disease Models, Animal
MH  - Fasciola hepatica/*immunology
MH  - Fascioliasis/immunology/pathology/*prevention & control
MH  - Female
MH  - Histocytochemistry
MH  - Lipopolysaccharides/*immunology
MH  - Liver/immunology/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microscopy
MH  - Th1 Cells/immunology
MH  - Vaccination/*methods
EDAT- 2011/09/29 06:00
MHDA- 2012/03/07 06:00
CRDT- 2011/09/28 06:00
PHST- 2011/09/28 06:00 [entrez]
PHST- 2011/09/29 06:00 [pubmed]
PHST- 2012/03/07 06:00 [medline]
AID - jir606 [pii]
AID - 10.1093/infdis/jir606 [doi]
PST - ppublish
SO  - J Infect Dis. 2012 Feb 1;205(3):506-14. doi: 10.1093/infdis/jir606. Epub 2011 Sep 
      23.