PMID- 21951393
OWN - NLM
STAT- MEDLINE
DCOM- 20120416
LR  - 20120105
IS  - 1167-1122 (Print)
IS  - 1167-1122 (Linking)
VI  - 21
IP  - 6
DP  - 2011 Nov-Dec
TI  - Functional and phenotypical alterations of polymorphonuclear cells in Sezary 
      syndrome patients.
PG  - 921-9
LID - 10.1684/ejd.2011.1544 [doi]
AB  - Sezary syndrome (SS), the leukemic variant of cutaneous T-cell lymphoma (CTCL), 
      has a poor prognosis and infections represent the most frequent cause of death. 
      Polymorphonucleate granulocytes (PMNs) constitute an essential part of the innate 
      immune system: their phagocytic and killing activity against pathogens is 
      mediated by the interactions between Toll-like receptors (TLRs) and the 
      Pathogen-associated molecular patterns (PAMPs). The aim of this study was to 
      investigate PMN functional activity and phenotype in SS patients and their 
      correlation with the onset of infectious complications. This prospective study 
      enrolled 18 consecutive SS patients; PMN functional activity was evaluated by 
      phagocytosis and intracellular killing tests towards Klebsiella pneumoniae. 
      Flow-cytometry was applied to analyze PMN phenotype. PMNs from SS patients 
      displayed a reduced phagocytic activity and intracellular killing against K. 
      pneumoniae at 30 min and 60 min, more pronounced in SS patients with recurrent 
      infections. CD11b and CD66b median fluorescence intensity (MFI) was significantly 
      higher in SS than in healthy subjects, whereas CD62L MFI was decreased. No 
      significant differences in TLR2, 4, 8 and 9 percentage expression or MFI were 
      found. An increased TLR5 percentage expression was documented. The impairment in 
      PMN functional activities in SS could favour the immune-suppression and raise 
      infection risk.
FAU - Fierro, Maria Teresa
AU  - Fierro MT
AD  - Department of Dermatology, Biomedical Sciences and Human Oncology, Turin 
      University, Via Cherasco 23, 10126 Turin, Italy.
FAU - Cuffini, Anna Maria
AU  - Cuffini AM
FAU - Novelli, Mauro
AU  - Novelli M
FAU - Banche, Giuliana
AU  - Banche G
FAU - Allizond, Valeria
AU  - Allizond V
FAU - Comessatti, Alessandra
AU  - Comessatti A
FAU - Brizio, Matteo
AU  - Brizio M
FAU - Scalas, Daniela
AU  - Scalas D
FAU - Merlino, Chiara
AU  - Merlino C
FAU - Quaglino, Pietro
AU  - Quaglino P
FAU - Bernengo, Maria Grazia
AU  - Bernengo MG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Dermatol
JT  - European journal of dermatology : EJD
JID - 9206420
RN  - 0 (Toll-Like Receptor 5)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Klebsiella pneumoniae/physiology
MH  - Male
MH  - Middle Aged
MH  - Neutrophils/*pathology/*physiology
MH  - Phagocytosis/physiology
MH  - Phenotype
MH  - Prospective Studies
MH  - Sezary Syndrome/immunology/mortality/*pathology/*physiopathology
MH  - Toll-Like Receptor 5/metabolism
MH  - Toll-Like Receptors/metabolism
EDAT- 2011/09/29 06:00
MHDA- 2012/04/17 06:00
CRDT- 2011/09/29 06:00
PHST- 2011/09/29 06:00 [entrez]
PHST- 2011/09/29 06:00 [pubmed]
PHST- 2012/04/17 06:00 [medline]
AID - ejd.2011.1544 [pii]
AID - 10.1684/ejd.2011.1544 [doi]
PST - ppublish
SO  - Eur J Dermatol. 2011 Nov-Dec;21(6):921-9. doi: 10.1684/ejd.2011.1544.