PMID- 21953690
OWN - NLM
STAT- MEDLINE
DCOM- 20121024
LR  - 20120521
IS  - 1097-0347 (Electronic)
IS  - 1043-3074 (Linking)
VI  - 34
IP  - 6
DP  - 2012 Jun
TI  - Composition and architecture of biofilms on used voice prostheses.
PG  - 863-71
LID - 10.1002/hed.21833 [doi]
AB  - BACKGROUND: Biofilms on medical devices are a frequent reason for failure of the 
      device. Voice prostheses in laryngectomized patients deteriorate within 3 to 4 
      months due to adhering biofilms, impeding proper functioning. Recently, we showed 
      that these biofilms are dominated by Candida and lactobacilli. However, the early 
      report of this finding lacked an in depth analysis of the species diversity and 
      community structure. METHODS: Denaturing gradient gel electrophoresis (DGGE) and 
      sequence analysis of amplified rRNA genes was used to identify microorganisms. 
      Fluorescence in situ hybridization (FISH) was used to analyze biofilm 
      architecture. RESULTS: Candida are the predominant fungi in the biofilms, while 
      lactobacilli are the predominant bacteria in all investigated biofilms, followed 
      by streptococci. FISH shows that lactobacilli and streptococci seem to have an 
      important interaction with fungi. CONCLUSION: The results give a better 
      understanding of biofilm formation and can be used in further development to 
      prevent biofilm formation on voice prostheses.
CI  - Copyright (c) 2011 Wiley Periodicals, Inc.
FAU - Buijssen, Kevin J D A
AU  - Buijssen KJ
AD  - Department of Biomedical Engineering, University Medical Center Groningen and 
      University of Groningen, Hanzeplein 1, P.O. Box 30001, 9700 RB Groningen, The 
      Netherlands.
FAU - van der Laan, Bernard F A M
AU  - van der Laan BF
FAU - van der Mei, Henny C
AU  - van der Mei HC
FAU - Atema-Smit, Jelly
AU  - Atema-Smit J
FAU - van den Huijssen, Pauline
AU  - van den Huijssen P
FAU - Busscher, Henk J
AU  - Busscher HJ
FAU - Harmsen, Hermie J M
AU  - Harmsen HJ
LA  - eng
PT  - Journal Article
DEP - 20110922
PL  - United States
TA  - Head Neck
JT  - Head & neck
JID - 8902541
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Fungal)
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - *Biofilms
MH  - DNA, Bacterial/isolation & purification
MH  - DNA, Fungal/isolation & purification
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Female
MH  - Gram-Negative Bacteria/physiology
MH  - Gram-Positive Bacteria/physiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Larynx, Artificial
MH  - Male
MH  - Microscopy, Confocal
MH  - Oligonucleotide Probes
MH  - Polymerase Chain Reaction
MH  - Prosthesis-Related Infections/*microbiology
EDAT- 2011/09/29 06:00
MHDA- 2012/10/25 06:00
CRDT- 2011/09/29 06:00
PHST- 2011/03/09 00:00 [revised]
PHST- 2011/04/28 00:00 [accepted]
PHST- 2011/09/06 00:00 [received]
PHST- 2011/09/29 06:00 [entrez]
PHST- 2011/09/29 06:00 [pubmed]
PHST- 2012/10/25 06:00 [medline]
AID - 10.1002/hed.21833 [doi]
PST - ppublish
SO  - Head Neck. 2012 Jun;34(6):863-71. doi: 10.1002/hed.21833. Epub 2011 Sep 22.