PMID- 21953869
OWN - NLM
STAT- MEDLINE
DCOM- 20120511
LR  - 20211020
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Print)
IS  - 1932-6254 (Linking)
VI  - 5
IP  - 9
DP  - 2011 Oct
TI  - Human umbilical cord mesenchymal stromal cells in a sandwich approach for 
      osteochondral tissue engineering.
PG  - 712-21
LID - 10.1002/term.370 [doi]
AB  - Cell sources and tissue integration between cartilage and bone regions are 
      critical to successful osteochondral regeneration. In this study, human umbilical 
      cord mesenchymal stromal cells (hUCMSCs), derived from Wharton's jelly, were 
      introduced to the field of osteochondral tissue engineering and a new strategy 
      for osteochondral integration was developed by sandwiching a layer of cells 
      between chondrogenic and osteogenic constructs before suturing them together. 
      Specifically, hUCMSCs were cultured in biodegradable poly-L-lactic acid scaffolds 
      for 3 weeks in either chondrogenic or osteogenic medium to differentiate cells 
      toward cartilage or bone lineages, respectively. A highly concentrated cell 
      solution containing undifferentiated hUCMSCs was pasted onto the surface of the 
      bone layer at week 3 and the two layers were then sutured together to form an 
      osteochondral composite for another 3 week culture period. Chondrogenic and 
      osteogenic differentiation was initiated during the first 3 weeks, as evidenced 
      by the expression of type II collagen and runt-related transcription factor 2 
      genes, respectively, and continued with the increase of extracellular matrix 
      during the last 3 weeks. Histological and immunohistochemical staining, such as 
      for glycosaminoglycans, type I collagen and calcium, revealed better integration 
      and transition of these matrices between two layers in the composite group 
      containing sandwiched cells compared to other control composites. These results 
      suggest that hUCMSCs may be a suitable cell source for osteochondral 
      regeneration, and the strategy of sandwiching cells between two layers may 
      facilitate scaffold and tissue integration.
CI  - Copyright (c) 2010 John Wiley & Sons, Ltd.
FAU - Wang, Limin
AU  - Wang L
AD  - Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 
      48109, USA.
FAU - Zhao, Liang
AU  - Zhao L
FAU - Detamore, Michael S
AU  - Detamore MS
LA  - eng
GR  - R21 DE017673/DE/NIDCR NIH HHS/United States
GR  - R21 DE017673-01/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20101230
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Aggrecans)
RN  - 0 (Antigens, Surface)
RN  - 0 (Collagen Type I)
RN  - 0 (Collagen Type II)
RN  - 0 (Glycosaminoglycans)
RN  - 9007-49-2 (DNA)
RN  - RMB44WO89X (Hydroxyproline)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Aggrecans/genetics/metabolism
MH  - Antigens, Surface/metabolism
MH  - Bone and Bones/*physiology
MH  - Calcium/metabolism
MH  - Cartilage/*physiology
MH  - Chondrogenesis
MH  - Collagen Type I/genetics/metabolism
MH  - Collagen Type II/genetics/metabolism
MH  - DNA/metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Gene Expression Regulation
MH  - Glycosaminoglycans/metabolism
MH  - Humans
MH  - Hydroxyproline/metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Osteogenesis
MH  - Tissue Engineering/*methods
MH  - Umbilical Cord/*cytology
PMC - PMC3770475
MID - NIHMS479605
EDAT- 2011/09/29 06:00
MHDA- 2012/05/12 06:00
PMCR- 2013/09/11
CRDT- 2011/09/29 06:00
PHST- 2010/01/22 00:00 [received]
PHST- 2010/08/31 00:00 [accepted]
PHST- 2011/09/29 06:00 [entrez]
PHST- 2011/09/29 06:00 [pubmed]
PHST- 2012/05/12 06:00 [medline]
PHST- 2013/09/11 00:00 [pmc-release]
AID - 10.1002/term.370 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2011 Oct;5(9):712-21. doi: 10.1002/term.370. Epub 2010 
      Dec 30.